CYBB Antikörper

Produktnummer ABIN1042596

Dieses Anti-CYBB-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CYBB in WB, IF, IHC (p) und ICC. Geeignet für Human. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für CYBB Antikörper (ABIN1042596)

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CYBB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Klon: 54-1

Produktdetails anti-CYBB Antikörper

Spezifität: This antibody is specific for NOX2/gp91phox 382-PKIAVDGP-389

Aufreinigung: Protein G

Immunogen: Partially Purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal antibody concentration should be determined by titration, however as a guideline try,WB: Use at an assay dependent dilution. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).ICC/IF: Use a concentration of 1 μg/mL.IHC-P: Use a concentration of 5 μg/mL. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol

Kommentare:

Myeloma, fusion partners: SP2/0

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Purified antibody containing PBS 0.1 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Referenzen für anti-CYBB Antikörper (ABIN1042596)

Baniulis, Nakano, Nauseef, Banfi, Cheng, Lambeth, Burritt, Taylor, Jesaitis: "Evaluation of two anti-gp91phox antibodies as immunoprobes for Nox family proteins: mAb 54.1 recognizes recombinant full-length Nox2, Nox3 and the C-terminal domains of Nox1-4 and cross-reacts with GRP 58." in: Biochimica et biophysica acta, Vol. 1752, Issue 2, pp. 186-96, (2005) (PubMed).

Burritt, Quinn, Jutila, Bond, Jesaitis: "Topological mapping of neutrophil cytochrome b epitopes with phage-display libraries." in: The Journal of biological chemistry, Vol. 270, Issue 28, pp. 16974-80, (1995) (PubMed).

Details zu CYBB

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Andere Bezeichnung: NOX2/gp91phox

Hintergrund: Synonyms:
CGD91-phox antibody,CYBB antibody,Cytochrome b(558) beta chain antibody,Cytochrome b-245 heavychain antibody,gp91-1 antibody,gp91-phox antibody,Heme-binding membrane glycoprotein gp91phox antibody,NADPHoxidase 2 antibody,Neutrophil cytochrome b 91 kDa polypeptide antibody,NOX2 antibody,p22 phagocyte B-cytochromeantibody,Superoxide-generating NADPH oxidase heavy chain subunit antibody

Gen-ID: 21577

OMIM: 300481

UniProt: Q01062