VCAM1 Antikörper (Vascular Cell Adhesion Molecule 1)

Details for Product anti-VCAM1 Antibody No. ABIN1042491
Antigen
  • CD106
  • INCAM-100
  • Vcam-1
  • VCAM1B
  • fi76d06
  • wu:fi76d06
  • zgc:158875
  • VCAM1
  • vascular cell adhesion molecule 1
  • vascular cell adhesion molecule 1b
  • VCAM1
  • Vcam1
  • vcam1b
  • VCAM-1
Reaktivität
Human
454
188
96
33
11
9
8
8
3
Wirt
Maus
331
179
93
16
13
8
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser VCAM1 Antikörper ist unkonjugiert
67
53
52
15
12
9
9
6
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
Applikation
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
340
236
205
156
138
102
58
46
42
26
26
24
17
13
10
5
3
3
2
1
1
Optionen
Klon 4B9
Isotyp IgG1
Spezifität The antibody binds exclusively to the cytokine-induced endothelial cell adhesion protein, vascular cell adhesion molecule-1 (VCAM-1).
Produktmerkmale Tumour marker
Reinigung Purified
Plasmids, Primers & others Plasmide, Primers & weitere VCAM1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung VCAM-1 (VCAM1 Antibody Abstract)
Hintergrund Vascular cell adhesion molecule-1 (VCAM-1) is induced on human umbilical vein endothelium (HUVE) by recombinant human tumor necrosis factor - alpha (rh TNF-alpha), rh interleukin-1 (IL-1) and lipopolysaccharide (LPS) and is involved in adherence of lymphocytic cell lines to HUVE. VCAM-1 is only minimally expressed on unstimulated endothelium. Molecular cloning and sequencing of VCAM-1 predicts a 69-kDa core protein with 6 potential N-linked glycosylation sites, if the protein is fully glycosylated a mature protein of about 90kDa is yielded. VCAM-1 mediates a component of the adherence of Peripheral Blood Lymphocytes (PBL) to rh TNF-stimulated HUVE, this CD18 independent mechanism of lymphocyte adherence to cytokine stimulated endothelium may be an important pathway of lymphocyte emigration at sites of inflammation and immune reaction.
Pathways Carbohydrate Homeostasis
Applikations-hinweise 4B9 is suitable for immunoblotting, immunoprecipitation, immunocytochemistry and immuno-histochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:25 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
Kommentare

4B9 is a mouse IgG1 monoclonal antibody generated by immunization of Balb/c mice with human umbilical vein endothelium (HUVE) that were non-enzymatically harvested after stimulation for 24h with recombinant human (rh) TNF-alpha (10 ng/ml). Splenocytes were fused with the NS-1 nonsecretory murine myeloma line.

Beschränkungen Nur für Forschungszwecke einsetzbar
Buffer PBS containing 0.09% sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Produkt verwendet in: Chuluyan, Osborn, Lobb, Issekutz: "Domains 1 and 4 of vascular cell adhesion molecule-1 (CD106) both support very late activation antigen-4 (CD49d/CD29)-dependent monocyte transendothelial migration." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 6, pp. 3135-4, 1995 (PubMed).

Pinola, Saksela, Tiisala, Renkonen: "Human NK cells expressing alpha 4 beta 1/beta 7 adhere to VCAM-1 without preactivation." in: Scandinavian journal of immunology, Vol. 39, Issue 2, pp. 131-6, 1994 (PubMed).

Bacchi, Marsh, Perkins, Carithers, McVicar, Hudkins, Benjamin, Harlan, Lobb, Alpers: "Expression of vascular cell adhesion molecule (VCAM-1) in liver and pancreas allograft rejection." in: The American journal of pathology, Vol. 142, Issue 2, pp. 579-91, 1993 (PubMed).

Ockenhouse, Tegoshi, Maeno, Benjamin, Ho, Kan, Thway, Win, Aikawa, Lobb: "Human vascular endothelial cell adhesion receptors for Plasmodium falciparum-infected erythrocytes: roles for endothelial leukocyte adhesion molecule 1 and vascular cell adhesion molecule 1." in: The Journal of experimental medicine, Vol. 176, Issue 4, pp. 1183-9, 1992 (PubMed).

Carlos, Schwartz, Kovach, Yee, Rosa, Osborn, Chi-Rosso, Newman, Lobb, Rosso: "Vascular cell adhesion molecule-1 mediates lymphocyte adherence to cytokine-activated cultured human endothelial cells." in: Blood, Vol. 76, Issue 5, pp. 965-70, 1990 (PubMed).

Haben Sie etwas anderes gesucht?