CD4 Antikörper

Produktnummer ABIN1042376

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-CD4 Antikörper wird verwendet zum Nachweis von CD4 in Proben von Human. Er wurde validiert für FACS, IHC, IF, Func, IHC (p), StM, Q, Diff und D.

Kurzübersicht für CD4 Antikörper (ABIN1042376)

Target: CD4

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD4 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Staining Methods (StM), Quantification (Q), Differentiation (Diff), Detection (D)

Klon: Edu-2

Produktdetails anti-CD4 Antikörper

Verwendungszweck: Mouse Anti Human CD4

Spezifität: CD4 (Edu-2) recognizes the CD4 antigen (a 55 kD glycoprotein).

Produktmerkmale: CD4 (Cluster of Differentiation 4) is a glycoprotein expressed on the surface of T helper cells, monocytes, macrophages, and dendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 (after the OKT4 monoclonal antibody that reacted with it) before being named CD4 in 1984. In humans, the CD4 protein is encoded by the CD4 gene. CD4 plays a role in the recognition of foreign antigens presented to T cells by MHC class II molecules. Furthermore, this antigen acts as a receptor for HIV-1 by binding the viral protein gp120. The CD4 antigen is present on most thymocytes and a subpopulation of peripheral blood T cells, called T helper cells (Th). In addition, CD4 is expressed on monocytes and weak on macrophages.

Immunogen: Edu-2 is a mouse monoclonal IgG2a antibody raised against CD4.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry and immunohistochemistry using frozen and paraffin embedded tissue sections. Optimal antibody dilution should be determined by titration. CD4 (Edu-2) is used in routine blood testing for CD4+ cells and CD4/CD8 ratios (e.g. HIV/AIDS patients) or as part of panels for the detection and differentiation of certain T cell leukemias. All these reagents are effectively formulated for indirect immunofluorescent staining of human blood for flow cytometric analysis using 10 μL/106 leukocytes. Staining with clone Edu-2 (CD4) monoclonal antibodies by flow cytometry analysis of normal blood cells is illustrated. Indirect staining was performed using 10 μL of the purified monoclonal antibody with RaM FITC conjugate and 100 μL blood sample. However each investigator should titer the antibody in their application to determine the optimal per-test amount. CD4 is also used in studies of functional activity of Th-cells in bacterial and viral infections, development of auto-immune diseases, transplant rejection, immune protection in response to allergens or allergenic reactivity.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Each vial contains 1 mL of 100 μg/mL purified monoclonal antibody in 0.01 M sodium phosphate, 0.15 M NaCl, 0.2 % BSA, 0.09 % sodium azide, pH 7.3.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Referenzen für anti-CD4 Antikörper (ABIN1042376)

Allison, Lanier: "Structure, function, and serology of the T-cell antigen receptor complex." in: Annual review of immunology, Vol. 5, pp. 503-40, (1987) (PubMed).

Kaneoka, Perez-Rojas, Sasasuki, Benike, Engleman: "Human T lymphocyte proliferation induced by a pan-T monoclonal antibody (anti-Leu 4): heterogeneity of response is a function of monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 1, pp. 158-64, (1983) (PubMed).

Friedrich, OReilly, Koziner, Gebhard, Good, Evans: "T-lymphocyte reconstitution in recipients of bone marrow transplants with and without GVHD: imbalances of T-cell subpopulations having unique regulatory and cognitive functions." in: Blood, Vol. 59, Issue 4, pp. 696-701, (1982) (PubMed).

Details zu CD4

Target: CD4

Andere Bezeichnung: CD4

UniProt: P01730

Pathways: T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events