CD4 Antikörper

Produktnummer ABIN1042376

Der Maus Monoklonal Anti-CD4-Antikörper wurde für FACS, IHC (fro), ICC und IHC (p) validiert. Er ist geeignet, CD4 in Proben von Human zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für CD4 Antikörper (ABIN1042376)

Target: CD4

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD4 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: Edu-2

Produktdetails anti-CD4 Antikörper

Spezifität: CD4 (Edu-2) recognizes the CD4 antigen (a 55 kD glycoprotein).

Produktmerkmale: CD markers

Aufreinigung: Purified

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry and immunohistochemistry using frozen and paraffin embedded tissue sections. Optimal antibody dilution should be determined by titration. CD4 (Edu-2) is used in routine blood testing for CD4+ cells and CD4/CD8 ratios (e.g. HIV/AIDS patients) or as part of panels for the detection and differentiation of certain T cell leukemias. CD4 is also used in studies of functional activity of Th-cells in bacterial and viral infections, development of auto-immune diseases, transplant rejection, immune protection in response to allergens or allergenic reactivity.

Kommentare:

Edu-2 is a mouse monoclonal IgG2a antibody raised against CD4.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 µg/mL

Buffer: 0.01 M sodium phosphate, 0.15 M NaCl, 0.2% BSA, 0.09% sodium azide, pH 7.3.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-CD4 Antikörper (ABIN1042376)

Allison, Lanier: "Structure, function, and serology of the T-cell antigen receptor complex." in: Annual review of immunology, Vol. 5, pp. 503-40, (1987) (PubMed).

Kaneoka, Perez-Rojas, Sasasuki, Benike, Engleman: "Human T lymphocyte proliferation induced by a pan-T monoclonal antibody (anti-Leu 4): heterogeneity of response is a function of monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 1, pp. 158-64, (1983) (PubMed).

Friedrich, OReilly, Koziner, Gebhard, Good, Evans: "T-lymphocyte reconstitution in recipients of bone marrow transplants with and without GVHD: imbalances of T-cell subpopulations having unique regulatory and cognitive functions." in: Blood, Vol. 59, Issue 4, pp. 696-701, (1982) (PubMed).

Details zu CD4

Target: CD4

Andere Bezeichnung: CD4

Hintergrund: CD4 (Cluster of Differentiation 4) is a glycoprotein expressed on the surface of T helper cells, monocytes, macrophages, and dendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 (after the OKT4 monoclonal antibody that reacted with it) before being named CD4 in 1984. In humans, the CD4 protein is encoded by the CD4 gene. CD4 plays a role in the recognition of foreign antigens presented to T cells by MHC class II molecules. Furthermore, this antigen acts as a receptor for HIV-1 by binding the viral protein gp120. The CD4 antigen is present on most thymocytes and a subpopulation of peripheral blood T cells, called T helper cells (Th). In addition, CD4 is expressed on monocytes and weak on macrophages.

Pathways: T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events