LPO Antikörper

Produktnummer ABIN103436

Hersteller Produkt- Nr.: 200-601-146-0100

Der Schaf Polyklonal Anti-LPO-Antikörper wurde für WB, IP und ELISA validiert. Er ist geeignet, LPO in Proben von Rind (Kuh) zu detektieren.

Kurzübersicht für LPO Antikörper (ABIN103436)

Target: LPO (Lactoperoxidase (LPO))

Reaktivität: Rind (Kuh)

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser LPO Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Produktdetails anti-LPO Antikörper

Hersteller Produkt- Nr.: 200-601-146-0100

Hersteller: Rockland

Verwendungszweck: Lactoperoxidase Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Lactoperoxidase [Bovine Milk].

Produktmerkmale: Synonyms: sheep anti-Lactoperoxidase Antibody, LPO antibody, Salivary peroxidase antibody, SAPX antibody, SPO antibody

Aufreinigung: Anti-LACTOPEROXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Lactoperoxidase [Bovine Milk]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Lactoperoxidase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Lactoperoxidase [Bovine Milk] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Rabbit) code #613-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu LPO

Target: LPO (Lactoperoxidase (LPO))

Andere Bezeichnung: Lactoperoxidase

Hintergrund: Background: Lactoperoxidase is an antimicrobial agent which utilizes hydrogen peroxide and thiocyanate (SCN) to generate the antimicrobial substance hypothiocyanous acid (HOSCN). It may protect the udder from infection and promote growth in newborn calves. It inhibits growth of the following bacterial species: E.coli, K.pneumoniae, P.aeruginosa, S.sonnei, S.saphrophyticus, S.epidermidis, and S.dysenteriae.

Gen-ID: 280844

UniProt: P80025