HMGN1 Antikörper

Produktnummer ABIN103313

Hersteller Produkt- Nr.: 200-301-278

Produktdetails anti-HMGN1 Antikörper

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HMGN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Hersteller Produkt- Nr.: 200-301-278

Hersteller: Rockland

Verwendungszweck: IPO-38 Antibody

Kreuzreaktivität (Details): This antigen was detected after 12h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes.

Produktmerkmale: Synonyms: mouse anti-IPO38 antibody, mouse anti-HMGN1 antibody, mouse anti-HMG14 antibody, Non-histone chromosomal protein HMG-14 antibody, High-mobility group nucleosome-binding domain-containing protein 1 antibody

Aufreinigung: Anti-IPO-38 monoclonal antibody was purified by Protein A chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: A BALB/c mouse was immunized with spleen cells of a human patient with hairy cell leukemia.

Immunogen Type: Native Protein

Klon: IPO-38

Isotyp: IgM kappa

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 10 μg/mL

Application Note: Anti-IPO-38 has been tested by immunohistochemistry (frozen and formalin/paraffin) and western blot analysis on the Raji cell line and reacts with a 14 -16 kDa protein. This antibody is suitable for ELISA and immunoprecipitation.  The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells (paraformaldehyde fixed, Triton X-100 permeabilized).  An increase of 400 % is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemid).  IPO-38 does not block the binding of the Ki-67 antibody.   Tonsillar tissue is typically used as a positive control.

Western Blot Dilution: 1:500- 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu HMGN1

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Andere Bezeichnung: Hmgn1 (HMGN1 Produkte)

Hintergrund: Background: The specificity of mAb IPO-38 was demonstrated in different human and murine lymphoid and non-lymphoid cell lines. It does not react with mononuclear cells and granulocytes of healthy donors but does react with nuclei of blood cells of patients with different forms of leukemia and with cells from lymph nodes of patients with Hodgkin's disease and non-hodgkin's lymphoma. Also a positive reaction in nuclei of breast and colorectal tumor cells was demonstrated. IPO-38 may be a good marker of cellular proliferation during monitoring of tumor progression.

Gen-ID: 15312

NCBI Accession: NP_032277

UniProt: P18608

Pathways: Chromatin Binding