Dieser Lambda-IgLC Antikörper ist konjugiert mit FITC
Applikation
Flow Cytometry (FACS)
Verwendungszweck
Anti-Hu Ig Lambda Light Chain FITC
Spezifität
The mouse monoclonal antibody 1-155-2 recognizes lambda light chains (22.5 kDa) of human immunoglobulin.
Kreuzreaktivität (Details)
Human
Aufreinigung
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Lambda-IgLC
Reaktivität: Ratte
WB, IF (cc), IF (p), ELISA, IHC (p), IHC (fro)
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Do not freeze. Avoid prolonged exposure to light.
Lagerung
4 °C
Informationen zur Lagerung
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Tierens, Holte, Warsame, Ikonomou, Wang, Chan, Delabie: "Low levels of monoclonal small B cells in the bone marrow of patients with diffuse large B-cell lymphoma of activated B-cell type but not of germinal center B-cell type." in: Haematologica, Vol. 95, Issue 8, pp. 1334-41, (2010) (PubMed).
Bahler, Pindzola, Swerdlow: "Splenic marginal zone lymphomas appear to originate from different B cell types." in: The American journal of pathology, Vol. 161, Issue 1, pp. 81-8, (2002) (PubMed).
Kubagawa, Gathings, Levitt, Kearney, Cooper: "Immunoglobulin isotype expression of normal pre-B cells as determined by immunofluorescence." in: Journal of clinical immunology, Vol. 2, Issue 4, pp. 264-9, (1983) (PubMed).
Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.,Immunoglobulin lambda, Igl