CD99 Antikörper (FITC)

Produktnummer ABIN1027669

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD99 in FACS. Er zeigt eine Reaktivität gegenüber Human und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für CD99 Antikörper (FITC) (ABIN1027669)

Target: CD99

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD99 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Klon: 3B2-TA8

Produktdetails anti-CD99 Antikörper (FITC)

Verwendungszweck: Anti-Hu CD99 FITC

Spezifität: The mouse monoclonal antibody 3B2/TA8 recognizes an extracellular epitope of CD99, an approximately 32 kDa sialoglycoprotein expressed on the surface of many cell types, with particularly strong expression on Ewing´s sarcoma and peripheral primitive neuroectodermal tumors. Within the hematopoietic system, CD99 is expressed on virtually all cell types except granulocytes.

Kreuzreaktivität (Details): Human

Aufreinigung: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human thymocytes

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD99 Antikörper (FITC) (ABIN1027669)

Brémond, Meynet, Mahiddine, Coito, Tichet, Scotlandi, Breittmayer, Gounon, Gleeson, Bernard, Bernard: "Regulation of HLA class I surface expression requires CD99 and p230/golgin-245 interaction." in: Blood, Vol. 113, Issue 2, pp. 347-57, (2009) (PubMed).

Kueng, Leb, Haiderer, Raposo, Thery, Derdak, Schmetterer, Neunkirchner, Sillaber, Seed, Pickl: "General strategy for decoration of enveloped viruses with functionally active lipid-modified cytokines." in: Journal of virology, Vol. 81, Issue 16, pp. 8666-76, (2007) (PubMed).

Waclavicek, Majdic, Stulnig, Berger, Sunder-Plassmann, Zlabinger, Baumruker, Stöckl, Ebner, Knapp, Pickl: "CD99 engagement on human peripheral blood T cells results in TCR/CD3-dependent cellular activation and allows for Th1-restricted cytokine production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 9, pp. 4671-8, (1998) (PubMed).

Details zu CD99

Target: CD99

Andere Bezeichnung: CD99

Hintergrund: CD99 Molecule (Xg blood group),CD99 is a ubiquitous transmembrane type I sialoglycoprotein of a unique and poorly characterized protein family. CD99 is heavily O-glycosylated and was described as a T cell costimulator and strong activator of integrin-mediated actin cytoskeleton assembly, promoting cell adhesion and homotypic aggregation, immediate arrest on an inflamed vascular endothelium, and cell migration through it. Ligation of CD99 under some conditions can lead to apoptosis. Originally CD99 was described as a human thymus leukemia antigen, an Ewing´s sarcoma-specific membrane marker, and an adhesion molecule involved in spontaneous rosette formation of T cells with erythrocytes.,MIC2, HBA71, blood group Xg

Gen-ID: 4267

UniProt: P14209