Kaninchen anti-Huhn IgG (Heavy & Light Chain) Antikörper - Preadsorbed

Details zu Produkt Nr. ABIN101033
Antigen
Epitop
Heavy & Light Chain
1571
134
101
57
34
33
25
6
6
6
6
5
4
4
4
3
3
1
1
1
Reaktivität
Huhn
356
343
337
234
233
122
113
87
79
76
72
67
61
57
55
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Wirt
Kaninchen
847
799
337
169
93
85
23
22
13
5
4
4
4
3
1
1
1
1
Klonalität
Polyklonal
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Optionen
Immunogen

Immunogen: Anti-Chicken IgG (H&L) was produced by repeated immunization with Chicken IgG whole molecule in rabbit.

Immunogen Type: Native Protein

Isotyp IgG
Spezifität IgG (H&L)
Kreuzreaktivität Huhn
Produktmerkmale Anti-Chicken IgG antibody generated in rabbit detects specifically chicken IgG. This secondary antibody anti-Chicken is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm
Reinigung Preadsorption: immunoaffinity chromatography using Chicken IgG coupled to agarose beads
Sterilität Sterile filtered
Antigen
Hintergrund

Synonyms: Rabbit anti-Chicken IgG Antibody, Chicken Secondary Antibody

Background: Anti-Chicken IgG Antibody generated in rabbit detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

Applikations-hinweise

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Chicken IgG (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

ELISA Dilution: 1:200,000 - 1:400,000

Western Blot Dilution: 1:10,000 - 1:30,000

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 10 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Haltbarkeit 12 months
Bilder des Herstellers
Image no. 1 for Rabbit anti-Chicken IgG (Heavy & Light Chain) antibody - Preadsorbed (ABIN101033) Western Blot of Anti-Chicken IgG (H&L) (RABBIT) Antibody . Lane M: 3 μl Molecular Lad...
Image no. 2 for Rabbit anti-Chicken IgG (Heavy & Light Chain) antibody - Preadsorbed (ABIN101033) Western Blot of Rabbit anti-Chicken IgG Antibody. Lane 1: Chicken IgG. Lane 2: None. ...