Ziege anti-Rind (Kuh) IgG (F(ab')2 Region) Antikörper (TRITC) - Preadsorbed

Details zu Produkt Nr. ABIN100919
Antigen
Epitop
F(ab')2 Region
1571
134
100
56
34
33
24
6
6
6
6
5
4
4
4
3
2
1
1
1
Reaktivität
Rind (Kuh)
355
342
337
233
232
122
113
87
80
72
72
65
61
57
54
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Wirt
Ziege
845
799
336
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Klonalität
Polyklonal
Konjugat
TRITC
236
220
209
150
146
130
75
49
48
35
31
26
26
26
26
26
25
23
23
23
22
22
21
21
21
20
20
20
20
20
20
18
16
14
13
10
9
9
9
9
8
8
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
Optionen
Immunogen

Immunogen: Bovine IgG F(ab')2 fragment

Isotyp IgG
Spezifität IgG F(ab')2
Produktmerkmale Concentration Definition: by UV absorbance at 280 nm
Reinigung Preadsorption: Solid phase absorption
Labeling Ratio 3.0
Antigen
Hintergrund

Synonyms: goat anti-Bovine IgG F(ab')2 fragment Rhodamine Conjugated Antibody, goat anti-Bovine IgG Fab2 fragment Rhodamine Conjugated Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.

Applikations-hinweise

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration 2.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Product is photosensitive and should be protected from light.
Lagerung RT,4 °C,-20 °C
Haltbarkeit 12 months