Hub1 Antikörper

Produktnummer ABIN100576

Hersteller Produkt- Nr.: 200-401-442

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Hub1 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Saccharomyces cerevisiae.

Kurzübersicht für Hub1 Antikörper (ABIN100576)

Target: Hub1 (Ubiquitin-Like Protein Modifier Hub1 (Hub1))

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Hub1 Antikörper

Hersteller Produkt- Nr.: 200-401-442

Hersteller: Rockland

Verwendungszweck: Hub1 Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Produktmerkmale: Synonyms: rabbit anti-Hub1 Antibody, rabbit anti-Hub 1 Antibody, Homologous to UBiquitin antibody, Hub1 protein antibody, HUB1 target protein 1 antibody, Hub1p antibody, Ubiquitin like protein modifier antibody

Aufreinigung: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast Hub1 protein.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This purified polyclonal antibody reacts with yeast Hub1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.   This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates).  Other intrinsic bands are readily detectable at lower dilutions. For immunoblotting a 9.7 kDa band corresponding to yeast Hub1 is detected.  Most yeast cell lysates can be used as a positive control without induction or stimulation. 

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 5.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu Hub1

Target: Hub1 (Ubiquitin-Like Protein Modifier Hub1 (Hub1))

Andere Bezeichnung: HUB1

Hintergrund: Background: Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin.  Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12.  Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs).  These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other.  In contrast to UBLs, UDPs are not conjugated to other proteins.  Hub1 (''Homologous to UBiquitin'') may function as a modifier (see figure 2) but its role is unclear because it lacks the double glycine motif characteristic for ubiquitin and ubiquitin-like modifiers. Recently cell polarity factors Sph1 and Hbt1 have been identified as in vivo targets of Hub1 conjugation.

Gen-ID: 855767, 6324360

UniProt: Q6Q546