AGO6 Antikörper
Kurzübersicht für AGO6 Antikörper (ABIN619533)
Target
Reaktivität
Wirt
Klonalität
Applikation
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Verwendungszweck
- Anti-AGO6 | Argonaute 6
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Kreuzreaktivität (Details)
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Arabidopsis thaliana
Not reactive in: Poplar sp., Zea mays -
Homologie
- Arabidopsis thaliana
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Aufreinigung
- Immunogen affinity purified serum
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Immunogen
- KLH-conjugated peptide derived from Arabidopsis thaliana AGO6 UniProt: O48771 TAIR:At2g32940
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Applikationshinweise
- 1 : 1000 (WB)
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Kommentare
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antibody binds microRNA and tasiRNAs, preference for 24nt siRNAs with 5'UAGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- For reconstitution add 50 μL of sterile water
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Buffer
- Immunogen affinity purified serum in PBS pH 7.4.
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Handhabung
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- AGO6 (Argonaute 6 (AGO6))
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Andere Bezeichnung
- AGO6
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Hintergrund
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Background: AGO6 (Argonaute 6) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
Additional information: There can be a reaction with NEB broad range prestained protein ladder depending upon secondary antibody used.AGO6 accumulates to lower levels in the nrpd1a mutant (Havecker at el. 2010). In addition, a non-specific band migrates to a similar location and thus protein was run on a 6 % denaturing gel to obtain good separation.If other secondary antibody is used than in the figure below, cross-reacting pattern can be different.
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Molekulargewicht
- 116.4 | 99 kDa
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Gen-ID
- 817856
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UniProt
- O48771
Target
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