Granzyme B Substrate 2-pNA

Produktnummer ABIN924953

Kurzübersicht für Granzyme B Substrate 2-pNA (ABIN924953)

Produktdetails

Verwendungszweck: Chromogenic paranitroanilide (pNA) peptide substrate for granzyme B.

Sequenz: Acetyl-Ile-Glu-Pro-Asp-paranitroa nilide, Ac-IEPD-pNA

Spezifität: Chromogenic substrate for granzyme B.

Reinheit: > 98 % by HPLC

Formel: C28H38N6O11

Anwendungsinformationen

Kommentare:

Soluble in DMSO and aqueous buffers. We recommend preparing a stock solution in high purity (>99.9%) DMSO, and diluting into aqueous buffer shortly prior to use.
1. Lyse cells in 50 mM Tris-HCl, pH 7.5, 0.3% NP-40, 1.0 mM DTT, at a density of 2 x 10^6 /mL.
2. Assay 0.01 mL cell lysate in a final volume of 0.1 mL. Assay buffer is cell lysis buffer containing 0.2 mM substrate.
3. Incubate at 37°C for 0-3 hrs. Take periodic readings of absorbance at 405 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Solid

Lagerung: 4 °C

Antigendetails

Hintergrund: Chromogenic paranitroanilide (pNA) peptide substrate for granzyme B. Release of free pNA is monitored by absorbance at 405 nm (epsilon=9,160 M^-1 cm^-1 ). Inhibitory data: Steady-state kinetic parameters for the hydrolysis of Ac-IEPD-pNA by recombinant granzyme B: Kcat = 4.16 S -1 Km x 10 -6 = 57 M Kcat/Km = 66,600 S -1 M -1.

Molekulargewicht: 634 Da