Human CD4 T Lymphocyte Enrichment Set-DM

Produktnummer ABIN2690970

Kurzübersicht für Human CD4 T Lymphocyte Enrichment Set-DM (ABIN2690970)

Target: CD4

Produktdetails

Reaktivität: Human

Marke: BD IMag™

Anwendungsinformationen

Kommentare:

BD IMag™ Human CD4 T Lymphocyte Enrichment Set-DM

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody or streptavidin was conjugated to the magnetic particles under optimum conditions, and unconjugated antibody/streptavidin was removed. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. 557939 Rev. 1 Page 1 of 3 Enrichment of CD4 T lymphocytes from human blood. PBMC were labeled with the BD IMag™ Human CD4 T Lymphocyte Enrichment Set - DM and separated on the BD IMagnet™ (Cat. No. 552311) according to the accompanying Protocol. To demonstrate the efficiency of the enrichment, cells were stained with APC-conjugated anti-human CD4 mAb RPA-T4 (Cat. No. 555349) to detect CD4 T lymphocytes and a mixture of PE-conjugated RPA-T8 (555367), ICRF44 (Cat. No. 555388), 3G8 (Cat. No. 555407), HIB19 (Cat. No. 555413), CB38 (NL07) (Cat. No. 555455), B159 (Cat. No. 555516), 7G3 (Cat. No. 554529), GA-R2 (HIR2) (Cat. No. 555570), and B1 (Cat. No. 555717) monoclonal antibodies to detect non-CD4 T leukocytes and erythrocytes. Dead cells were excluded by staining with propidium iodide. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system. Please refer to the Enrichment Flow Chart on the next page to identify the cell populations represented in this figure. The percentage of CD4 T cells is indicated in the lower-right corner of each panel. Far left panel shows unseparated PBMC. Middle left panel shows the combined enriched fraction after three 6-minute magnetic separations. Middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in top right panel. This additional separation step can result in up to 2-6% increased purity with less than a 5% decrease in recovery. Far right panel shows the positive fraction.

Antigendetails

Target: CD4

Andere Bezeichnung: CD4