Biotin Maus Lineage Panel

Produktnummer ABIN2690963

Kurzübersicht für Biotin Maus Lineage Panel (ABIN2690963)

Applikation: Flow Cytometry (FACS)

Produktdetails

Reaktivität: Maus

Konjugat: Biotin

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

BD Pharmingen™ Biotin Mouse Lineage Panel - Reactivity Ms

Probenmenge: 2 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. 559971 Rev. 2 Page 1 of 3 Depletion of lineage-committed cells from mouse bone marrow. BALB/c bone-marrow cells were labeled with BD™ Mouse Lineage Panel and BD™ IMag Streptavidin Particles Plus - MSC (Cat. no. 557811, at 10 μl per 1 x 10^7 total cells). The labeled cells were then separated over a magnetic separation column designed for depletions. To demonstrate the efficiency of the depletion, unmanipulated bone-marrow cells and the lineage-negative fraction were stained with APC-conjugated anti-mouse CD117 mAb 2B8 (Cat. No. 553356) to detect hematopoietic progenitors, and with PE-conjugated anti-mouse TER-119/Erythroid Cells (Cat. No. 553673), PE-conjugated anti-mouse CD45R/B220 (Cat. No. 553089/553090), and PE-conjugated anti-mouse CD11b (Cat. No. 557397/553311) to detect lineage-committed cells. The lineage-negative fraction contains a greatly increased proportion of CD117+ cells and less than 5% of lineage-positive contaminants.

Referenzen

Sato, Laver, Ogawa: "Reversible expression of CD34 by murine hematopoietic stem cells." in: Blood, Vol. 94, Issue 8, pp. 2548-54, (1999) (PubMed).

Goodell, Rosenzweig, Kim, Marks, DeMaria, Paradis, Grupp, Sieff, Mulligan, Johnson: "Dye efflux studies suggest that hematopoietic stem cells expressing low or undetectable levels of CD34 antigen exist in multiple species." in: Nature medicine, Vol. 3, Issue 12, pp. 1337-45, (1998) (PubMed).

Morrison, Wandycz, Hemmati, Wright, Weissman: "Identification of a lineage of multipotent hematopoietic progenitors." in: Development (Cambridge, England), Vol. 124, Issue 10, pp. 1929-39, (1997) (PubMed).

Spangrude, Scollay: "A simplified method for enrichment of mouse hematopoietic stem cells." in: Experimental hematology, Vol. 18, Issue 8, pp. 920-6, (1990) (PubMed).

Spangrude, Heimfeld, Weissman: "Purification and characterization of mouse hematopoietic stem cells." in: Science (New York, N.Y.), Vol. 241, Issue 4861, pp. 58-62, (1988) (PubMed).