Anti-DYKDDDDK G1 Affinity Resin

Produktnummer ABIN2017617

Produktdetails

Target: DYKDDDDK Tag

Reaktivität: Tag

Klonalität: Monoklonal

Applikation: Immunoprecipitation (IP), Purification (Purif), Separation (Sep)

Spezifität: High DYKDDDDK-tag binding capacity and ultralow levels of non-specific binding
Multiple elution methods for diverse protein characteristics and different requirements
Used for purification and immunoprecipitation of DYKDDDDK-tagged protein or protein complex

Produktmerkmale: DYKDDDDK tag is an octapeptide tag that can be added to a protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of a target protein to facilitate detection and purification. Anti-DYKDDDDK G1 Affinity Resin is designed for the purification of DYKDDDDK-tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells. The DYKDDDDK-tagged protein in the cell lysate can specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin. Nonspecific bindings are eliminated by stringent washing steps, and the target protein is finally eluted with high recovery rate.
Matrix: 4 % cross-linked agarose
Average bead size: 90 μm
Ligand: Mouse monoclonal antibody against DYKDDDDK tag, clone G1
Binding capacity: Approximately 1 mg DYKDDDDK-tagged protein per ml settled resin

Anwendungsinformationen

Kommentare:

Resin reuse: The resin can be recycled for at least 4 times. If maintained properly, the resin can be reused 10 times with minimum loss of binding capability.
Elution method: Acid, alkaline, neutral, peptide competitive and SDS-PAGE loading buffer elutions
Reagents compatibility: Compatible with commonly used reagents at certain concentrations

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 50 % settled resin in TBS with 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze the resin.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C for up to 12 months.

Haltbarkeit: 12 months

Antigendetails

Target: DYKDDDDK Tag

Andere Bezeichnung: DYKDDDDK (DYKDDDDK Tag Produkte)

Substanzklasse: Tag