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Maus TrueBlot® Anti-Maus Ig Biotin

Rockland
Produktnummer ABIN1589968
Hersteller Produkt- Nr.: 13-8817-82

Kurzübersicht für Maus TrueBlot® Anti-Maus Ig Biotin (ABIN1589968)

Applikation

Immunoprecipitation (IP), Western Blotting (WB)
  • Reaktivität

    Maus

    Wirt

    Ratte

    Klonalität

    Monoklonal

    Konjugat

    Biotin

    Hersteller Produkt- Nr.

    13-8817-82

    Hersteller

    Rockland

    Marke

    TrueBlot®

    Produktmerkmale

    Mouse IgG TrueBlot® ULTRA is a unique anti-mouse IgG immunoblotting (second step) reagent. Mouse IgG TrueBlot® ULTRA enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western blotting with Mouse IgG TrueBlot® ULTRA, simply substitute the conventional anti-Mouse IgG blotting reagent with Mouse IgG TrueBlot® ULTRA and follow the prescribed protocol for sample preparation and immunoblotting. The Biotin Mouse TrueBlot® ULTRA is the biotinylated format of Mouse IgG TrueBlot® ULTRA. It can be used as a secondary antibody, followed by the use of avidin-peroxidase conjugated (A003-03).
    Conjugation Name: Biotin TrueBlot® ULTRA

    Bestandteile

    Mouse TrueBlot®: Anti-Mouse Ig Biotin
  • Applikationshinweise

    Western Blot Dilution: 1:2000

    Kommentare

    Biotin Mouse TrueBlot® ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. Biotin Mouse TrueBlot® ULTRA preferentially detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Biotin Mouse TrueBlot® ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Note: There are two key procedural considerations:
    1. When using any TrueBlot® reagent, ensure there is complete reduction of the lysate.
    2. Use milk powder for complete and effective blocking of the western blot.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.5 mg/mL

    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: Proprietary

    0.09 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Do not freeze. Dilute only prior to immediate use.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store vial at 4 °C before opening. This product is stable at 4 °C as an undiluted liquid.

    Haltbarkeit

    6 months
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