Nucleophosmin/nucleoplasmin 2 Proteine (NPM2)

Human Nucleophosmin/nucleoplasmin 2 (NPM2) Interaktionspartner

1. Studies indicate that histone chalerones nucleoplasmin (NPM2/NPM3) preferentially associated with histones H2A-H2B in the egg and the nuclear autoantigenic sperm protein (NASP) families.

2. Characterization of the sperm chromatin decondensation and nucleosome assembly activities of homo- and hetero-oligomers of NPM1,NPM2 and NPM3.

3. The crystal structure of a truncated Npm2-core shows that the N-terminal domains of Npm2 and Np form similar pentamers, providing insights into the mechanism of histone binding by nucleoplasmins.

4. Expression of NPM2 in HeLa cells

5. Promoter methylation and differential gene expression of five markers: COL1A2, NPM2, HSPB6, DDIT4L and MT1G were validated by sequencing of bisulfite-modified DNA and real-time reverse transcriptase PCR, respectively.

Mouse (Murine) Nucleophosmin/nucleoplasmin 2 (NPM2) Interaktionspartner

1. Findings demonstrate that the NPM2-based oocyte nucleolus is an essential platform for parental chromatin organization in early embryonic development.

2. involved in sperm chromatin remodeling

3. NPM2 targeting of nucleolus-like bodies (NLBs) is regulated by the K-rich motif and is essential for the formation of NLBs.

4. Npm2 is a maternal effect gene critical for nuclear and nucleolar organization and embryonic development

5. Promoter activities of three mouse maternal genes (H1oo, Npm2, and Zar1) in oocytes and pre-implantation embryos, and examined the promoters and to obttain the best promoter for oocytte gene expression.

6. The highest Npm2 mRNA levels were detected in oocytes in the pre-antral follicle stage.