AAV5 VP1 Antikörper (Alexa Fluor 594)
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- Target
- AAV5 VP1 (Adeno-Associated Virus 5, VP1 (AAV5 VP1))
- Reaktivität
- Virus
- Wirt
- Kaninchen
- Klonalität
- Polyklonal
- Konjugat
- Dieser AAV5 VP1 Antikörper ist konjugiert mit Alexa Fluor 594
- Applikation
- Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- Spezifität
- This antibody will recognize many AAV strains, including AAV5, AAV4, AAV3B, AAV9, and AAV13
- Kreuzreaktivität
- Virus
- Kreuzreaktivität (Details)
- AAV5
- Aufreinigung
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from Adeno-Associated Virus 5 capsid protein VP1
- Isotyp
- IgG
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- Applikationshinweise
- IF(IHC-P) 1:50-200
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- AAV5 VP1 (Adeno-Associated Virus 5, VP1 (AAV5 VP1))
- Andere Bezeichnung
- AAV5 Capsid Protein VP1
- Substanzklasse
- Viral Protein
- Hintergrund
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Synonyms: capsid protein Adeno-associated 5 virus, capsid protein [Adeno-associated virus - 5], capsid protein AAV5, Parvovirus coat protein VP1, capsid protein.
Background: Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus
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