NACA Protein (AA 1-215) (His tag)

Produktnummer ABIN667605

Dieses Recombinant NACA-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für NACA Protein (AA 1-215) (His tag) (ABIN667605)

Target: NACA (NACa1) (Nascent Polypeptide Associated Complex alpha 1 (NACa1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 95 %

Produktdetails

Proteineigenschaft: AA 1-215

Aufreinigungstag / Konjugat: Dieses NACA Protein ist gelabelt mit His tag.

Sequenz: MPGEATETVP ATEQELPQPQ AETGSGTESD SDESVPELEE QDSTQATTQQ AQLAAAAEID EEPVSKAKQS RSEKKARKAM SKLGLRQVTG VTRVTIRKSK NILFVITKPD VYKSPASDTY IVFGEAKIED LSQQAQLAAA EKFKVQGEAV SNIQENTQTP TVQEESEEEE VDETGVEVKD IELVMSQANV SRAKAVRALK NNSNDIVNAI MELTM

Produktmerkmale: NACA, 1-215 aa, Human, His tag E.coli

Aufreinigung: > 95% by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 0.15M NaCl

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: NACA (NACa1) (Nascent Polypeptide Associated Complex alpha 1 (NACa1))

Andere Bezeichnung: NACA

Hintergrund: NACA is member of the nascent polypeptide associated complex (NAC) alpha subunit family that participate in preventing inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Most NACA proteins localize to both nucleus as well as cytoplasm, and contain NAC-A/B (NAC-alpha/beta) and uBA (ubiquitin-associated) domains. The uBA domain is associated with proteins involved in the ubiquitin-proteasome pathway for protein degradation. Recombinant human NACA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 25.5 kDa (235aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

NCBI Accession: NP_001106672

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development