IDH1 Protein (AA 1-414) (His tag)

Produktnummer ABIN667603

Recombinant IDH1-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für IDH1 Protein (AA 1-414) (His tag) (ABIN667603)

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Reinheit: > 95 %

Produktdetails

Proteineigenschaft: AA 1-414

Aufreinigungstag / Konjugat: Dieses IDH1 Protein ist gelabelt mit His tag.

Sequenz: MSKKISGGSV VEMQGDEMTR IIWELIKEKL IFPYVELDLH SYDLGIENRD ATNDQVTKDA AEAIKKHNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR NILGGTVFRE AIICKNIPRL VSGWVKPIII GRHAYGDQYR ATDFVVPGPG KVEITYTPSD GTQKVTYLVH NFEEGGGVAM GMYNQDKSIE DFAHSSFQMA LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSQFE AQKIWYEHRL IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLVCPDG KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWTRGL AHRAKLDNNK ELAFFANALE EVSIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK LGENLKIKLA QAKL

Produktmerkmale: IDH1, 1-414aa , Human, His tag, E.coli

Aufreinigung: > 95% by SDS-PAGE

Biological Activity Comment: Specific activity is > 18,000pmol/min/ug. One unit will oxidize 1.0pmole of DL-Isocitrate to D alpha-Ketoglutarate per minute in the presence of beta-NADP at pH7.4 at 37C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 0.1M NaCl,1 mM DTT,0.1 mM PMSF

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Andere Bezeichnung: Isocitrate Dehydrogenase 1/IDH1

Hintergrund: IDH1, also known as isocitrate dehydrogenase (IDHC) cytoplasmic enzyme, belongs to the isocitrate and isopropylmalate dehydrogenases family. This protein catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming alpha-ketoglutarate and CO2 in a two step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of alpha-ketoglutarate. Recombinant human IDH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Molekulargewicht: 48.8 kDa (434aa) confirmed by MALDI-TOF

NCBI Accession: NP_005887

Pathways: Warburg Effekt