NAPA Protein (AA 1-295) (His tag)
Kurzübersicht für NAPA Protein (AA 1-295) (His tag) (ABIN667207)
Target
Alle NAPA Proteine anzeigenProtein-Typ
Spezies
Quelle
Applikation
Reinheit
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Proteineigenschaft
- AA 1-295
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Aufreinigungstag / Konjugat
- Dieses NAPA Protein ist gelabelt mit His tag.
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Sequenz
- MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN MFKMAKNWSA AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA INCLMRAIEI YTDMGRFTIA AKHHISIAEI YETELVDIEK AIAHYEQSAD YYKGEESNSS ANKCLLKVAG YAALLEQYQK AIDIYEQVGT NAMDSPLLKY SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE CKLMKKLLEA HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR
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Produktmerkmale
- NAPA, 1-295aa, Human, His tag, E.coli
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Aufreinigung
- > 95% by SDS-PAGE
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N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 1-295) protein (His tag)
custom-made NAPA Spezies: Human Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 1-295) protein (His tag)custom-made NAPA Spezies: Maus Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 1-295) protein (GST tag)NAPA Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 1-295) protein (His-IF2DI Tag)NAPA Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 7.5) containing 10 % glycerol
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Handhabung
- Avoid repeated freezing and thawing cycles
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))
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Andere Bezeichnung
- NAPA
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Hintergrund
- NAPA is one of the SNAP (Soluble NSF Attachment Protein) family. SNAPs, acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. This protein mediates the binding of NSF and thus the formation of a 20S fusion particle. It is expressed in all mammalian tissues. Recombinant human NAPA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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Molekulargewicht
- 35.3 kDa (315aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_003818
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Pathways
- Synaptic Vesicle Exocytosis, Asymmetric Protein Localization
Target
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