AKR1C1 Protein (AA 1-323) (His tag)

Produktnummer ABIN666955

Recombinant AKR1C1-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für AKR1C1 Protein (AA 1-323) (His tag) (ABIN666955)

Target: AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Reinheit: > 90 %

Produktdetails

Proteineigenschaft: AA 1-323

Aufreinigungstag / Konjugat: Dieses AKR1C1 Protein ist gelabelt mit His tag.

Sequenz: MGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY

Produktmerkmale: AKR1C1, 1-323aa, Human, His tag, E.coli

Aufreinigung: > 90% by SDS-PAGE

Biological Activity Comment: Specific activity is > 500pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 20 % glycerol

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Andere Bezeichnung: Aldo-keto Reductase 1C1/AKR1C1

Hintergrund: AKR1C1 is member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. Recombinant AKR1C1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 38.9 kDa (343aa) confirmed by MALDI-TOF

NCBI Accession: NP_001344

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process