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SFRP5 ELISA Kit

SFRP5 Reaktivität: Human Colorimetric Sandwich ELISA 1.56-100 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN457072
  • Target Alle SFRP5 ELISA Kits anzeigen
    SFRP5 (Secreted Frizzled-Related Protein 5 (SFRP5))
    Reaktivität
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    1.56-100 pg/mL
    Untere Nachweisgrenze
    1.56 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    For the quantitative determination of human secreted frizzled-related protein 5 (SFRP5) concentrations in serum, plasma, cell culture supernates and tissue homogenates.
    Proben
    Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of human SFRP5.
    Kreuzreaktivität (Details)
    Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.
    Sensitivität
    0.39 pg/mL
    Bestandteile
    • Assay plate (12 × 8 coated Microwells)
    • Standard (freeze dried)
    • Biotin-antibody (100 × concentrate)
    • HRP-avidin (100 × concentrate)
    • Biotin-antibody Diluent
    • HRP-avidin Diluent
    • Sample Diluent
    • Wash Buffer (25 × concentrate)
    • TMB Substrate
    • Stop Solution
    • Adhesive Strip (for 96 wells)
    • Instruction manual
    Featured
    Zu unserem meistverkauften SFRP5 ELISA Kit
  • Applikationshinweise
    • The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
    • Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
    • Grossly hemolyzed samples are not suitable for use in this assay.
    • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
    • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
    • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
    • Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
    • Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
    • Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
    Kommentare

    Detection wavelength: 450 nm

    Information on standard material:
    Depending on the antigen to be detected, standards can be either native or recombinant protein. The recombinant proteins are being expressed in CHO cells in most cases. Please inquire for more information. The formulation of auxiliary material in the standard is considered proprietary information, however it does not contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

    Information on reagents:
    In most cases the stop solution provided is 1 N H2SO4. The formulation of wash solution is proprietary information. None of the components contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

    Information on antibodies:
    The antibodies provided in different kits vary in regards to clonality and host. Some antibodies are affinity purified, some are Protein A

    Probenmenge
    100 μL
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SFRP5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFRP5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
    Aufbereitung der Reagenzien
    • Biotin-antibody (1×) - Centrifuge the vial before opening.
      Biotin-antibody requires a 100-fold dilution. The suggested dilution is 10µL of Biotin-antibody + 990µL of Biotin-antibody Diluent.
    • HRP-avidin (1×) - Centrifuge the vial before opening.
      HRP-avidin requires a 100-fold dilution. The suggested dilution is 10µL of HRP-avidin + 990µL of HRP-avidin Diluent.
    • Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20mL of Wash Buffer Concentrate (25×) into deionized or distilled water to prepare 500mL of Wash Buffer (1×).
    • Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.
      Reconstitute the Standard with 1ml of Sample Diluent. Do not substitute other diluents. This reconstitution produces a stock solution. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
      Pipette 250µL of Sample Diluent into each tube. Use the stock solution to produce a 2-fold dilution series. Mix each tube thoroughly before the next transfer. The undiluted Standard serves as the high standard. Sample Diluent serves as the zero standard (0ng/mL).
    Note:
    • Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit.
    • Bring all reagents to room temperature (18-25°C) before use for 30 min.
    • Prepare fresh standard for each assay. Use within 4 hours and discard after use.
    • Making serial dilution in the wells directly is not permitted.
    • Please carefully reconstitute Standards according to the instruction. Avoid foaming and mix gently until the crystals have completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10µL when pipetting.
    • It is recommended to use distilled water to prepare reagents and samples. Using contaminated water or container for reagent preparation will influence detection result.
    Testpräzision
    Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
    Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
    • Intra-assay: CV% less than 8%
    • Inter-assay: CV% less than 10%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #029856 (ELISA)
    'Independent Validation' Siegel
    by
    Affina Biotechnologies, Inc
    No.
    #029856
    Datum
    05.04.2016
    Antigen
    Human secreted frizzled-related protein 5 (SFRP5)
    Chargennummer
    V23182624
    Validierte Anwendung
    ELISA
    Positivkontrolle
    Human mixed serum (Biochemed, Lot#BC033016HSPMG)
    Negativkontrolle
    Chicken serum (Biochemed, Lot#BC03316CSPMG)
    Bewertung
    Human SFRP5 was detected in the positive samples at ~4ng/mL and was not present in the negative control. Spike controls were recovered 100%.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    Sekundärantikörper
    Full Protocol
    • 1. 100 μL of standard and samples were added 96-well strip plates provided in the kit. All samples and standards were assayed in duplicate.
    • 2. The microplate was covered and incubated at 37°C for 2 hr.
    • 3. Plate contents were discarded.
    • 4. 100 μL of biotin antibody conjugate was added and incubated at 37°C for 1 hr. 5. Content of the wells was discarded and wells were washed 3 times with 200 μl of 1x wash solution
    • 6. 100 μL of HRP-avidin conjugate was added and incubated at 37°C for 1 hr.
    • 7. Contents of the wells were discarded and wells were washed 5 times with 300 μl of 1x wash solution.
    • 8. 90 μl of TMB substrate was added to each well. The plate was covered and incubated at 37°C for 15 min.
    • 9. 50 μl of the Stop Solution was added per well.
    • 10. The optical density (OD value) of each well was read immediately using a microplate reader set to 450 nm.
    • 11. The duplicate readings for each sample were averaged and the average zero standard optical density subtracted. The corrected average-value was tabulated as Average Absorbance. A standard curve was generated by plotting the mean optical density (OD) value for each standard on the x-axis against the concentration on the Y-axis using CurveExpert 1.4 (CUSABIO). A logistic function equation was used for the best fit through the points on the graph.
    • 12. The CurveExpert Analyze feature was used to calculate human SFRP5 concentrations of the samples based on their Average Absorbance values.
    Anmerkungen
    - The concentration of human SFRP5 in human and chicken sera was measured according to the manufacturer’s directions.
  • Vorsichtsmaßnahmen
    The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
    Handhabung
    • The kit should not be used beyond the expiration date on the kit label.
    • Do not mix or substitute reagents with those from other lots or sources.
    • If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
    • Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
    • This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    For unopened kit: All the reagents should be kept according to the labels on vials.
    Haltbarkeit
    6 months
  • Bougault, Briolay, Boutet, Pilet, Delplace, Le Goff, Guicheux, Blanchard, Magne: "Wnt5a is expressed in spondyloarthritis and exerts opposite effects on enthesis and bone in murine organ and cell cultures." in: Translational research : the journal of laboratory and clinical medicine, (2015) (PubMed).

    Qu, Wang, Deng, Wei, Deng: "Associations between longer habitual day napping and non-alcoholic fatty liver disease in an elderly Chinese population." in: PLoS ONE, Vol. 9, Issue 8, pp. e105583, (2014) (PubMed).

    Pham, Hawa, Roden, Schernthaner, Pozzilli, Buzzetti, Scherbaum, Seissler, Hunter, Leslie, Kolb, Schloot et al.: "Increased serum concentrations of adhesion molecules but not of chemokines in patients with Type 2 diabetes compared with patients with Type 1 diabetes and latent autoimmune diabetes in adult age: ..." in: Diabetic medicine : a journal of the British Diabetic Association, Vol. 29, Issue 4, pp. 470-8, (2012) (PubMed).

  • Target Alle SFRP5 ELISA Kits anzeigen
    SFRP5 (Secreted Frizzled-Related Protein 5 (SFRP5))
    Abstract
    SFRP5 Produkte
    Synonyme
    SARP3 ELISA Kit, AI605071 ELISA Kit, D144 ELISA Kit, frp1 ELISA Kit, zgc:101849 ELISA Kit, Sfrp5 ELISA Kit, XsFRP5 ELISA Kit, frp5 ELISA Kit, sFRP-5 ELISA Kit, sarp3 ELISA Kit, sfrp5 ELISA Kit, sfrp5-a ELISA Kit, sfrp5-b ELISA Kit, secreted frizzled related protein 5 ELISA Kit, secreted frizzled-related sequence protein 5 ELISA Kit, secreted frizzled-related protein 5 ELISA Kit, secreted frizzled-related protein 5 L homeolog ELISA Kit, secreted frizzled-related protein 5 S homeolog ELISA Kit, SFRP5 ELISA Kit, Sfrp5 ELISA Kit, sfrp5 ELISA Kit, sfrp5.L ELISA Kit, sfrp5.S ELISA Kit
    Hintergrund
    Synonyms: SARP3, secreted apoptosis related protein 3
    HGNC
    10779
    UniProt
    Q5T4F7
    Pathways
    WNT Signalweg
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