HGF ELISA Kit

Produktnummer ABIN411278

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human HGF. Es wurde in 4 Publikationen zitiert.

Kurzübersicht für HGF ELISA Kit (ABIN411278)

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Bindungsspezifität: AA 32-494, AA 495-728

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 125-8000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (citrate)

Produktdetails HGF ELISA Kit

Untere Nachweisgrenze: 125 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human HGF

Analytische Methode: Quantitative

Spezifität: Expression system for standard: sf21
Immunogen sequence: Q32-R494(alpha)&V495-S728(beta)

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <5pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: sf21
Immunogen sequence: Q32-R494(alpha)&V495-S728(beta)

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the peptidase S1 family. Plasminogen subfamily.

Plattentyp: Pre-coated

Protokoll: human HGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HGF has been precoated onto 96-well plates. Standards(sf21, Q32-R494( alpha) & V495-S728( beta)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human HGF amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 8000pg/mL,4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human HGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human HGF standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 521, Standard deviation: 39.6, CV(%): 7.6
• Sample 2: n=16, Mean(pg/ml): 3649, Standard deviation: 222.6, CV(%): 6.1
• Sample 3: n=16, Mean(pg/ml): 5621, Standard deviation: 269.8, CV(%): 4.8,
• Sample 1: n=24, Mean(pg/ml): 532, Standard deviation: 40.96, CV(%): 7.7
• Sample 2: n=24, Mean(pg/ml): 3576, Standard deviation: 303.96, CV(%): 8.5
• Sample 3: n=24, Mean(pg/ml): 5618, Standard deviation: 348.3, CV(%): 6.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für HGF ELISA Kit (ABIN411278)

Li, Hu, Wang, Zhang, Zhou, Yang, Li, Xiong, Liu, Li, Wu, Zheng: "Autophagy-dependent generation of Axin2+ cancer stem-like cells promotes hepatocarcinogenesis in liver cirrhosis." in: Oncogene, Vol. 36, Issue 48, pp. 6725-6737, (2017) (PubMed).

Maraldi, Beretti, Guida, Zavatti, De Pol: "Role of hepatocyte growth factor in the immunomodulation potential of amniotic fluid stem cells." in: Stem cells translational medicine, Vol. 4, Issue 6, pp. 539-47, (2015) (PubMed).

Guo, Sun, Xu, Zhao, Peng, Wang: "Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms." in: Neural regeneration research, Vol. 10, Issue 4, pp. 651-8, (2015) (PubMed).

Secchiero, Corallini, Zavan, Tripodo, Vindigni, Zauli: "Mesenchymal stem cells display hepato-protective activity in lymphoma bearing xenografts." in: Investigational new drugs, Vol. 30, Issue 2, pp. 803-7, (2012) (PubMed).

Details zu HGF

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Andere Bezeichnung: HGF

Hintergrund:

Protein Function: Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. .

Background: Hepatocyte growth factor(HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass(Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.

Synonyms: Hepatocyte growth factor,Hepatopoietin-A,Scatter factor,SF,Hepatocyte growth factor alpha chain,Hepatocyte growth factor beta chain,HGF,HPTA,

Full Gene Name: Hepatocyte growth factor

Gen-ID: 3082

UniProt: P14210

Pathways: RTK Signalweg, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor