Brain-Derived Neurotrophic Factor (BDNF) ELISA Kit

Antigen: Brain-Derived Neurotrophic Factor (BDNF)

Synonyme: MGC34632, MGC105254, bdnf-A, BDNF, MGC133995, bdnf, Bdnf

Reaktivität: Maus

Applikation: ELISA

Produktnummer: ABIN411259

Produktbeschreibung

Produktmerkmale: For quantitative detection of mouse BDNF in sera, plasma, body fluids, tissue lysates or cell culture supernates.

Weitere Bezeichnung: Brain derived neurotrophic facor (BDNF)

Beschreibung: Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system.1 BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically.2 BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system.3 BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state.4 It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury.5 The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13. 6

Spezifität: No detectable cross-reactivity with any other cytokine.

Sensitivität: Sensitivity: < 2pg/ml. Range: 31.2pg/ml-2000pg/ml

Anwendungen

Prinzip: This mouse BDNF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse BDNF specific-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection monoclonal antibodies (clone No. 35928.11) were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BDNF amount of sample captured in plate.

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing. Don

Bestandteile: Lyophilized recombinant mouse BDNF standard: 10ng/tube

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl, 450

Lagerung: Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) Four months at 4°C and eight months at -20°C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Brain-Derived Neurotrophic Factor (BDNF) ELISA Kit