GAPDHS Antikörper
Kurzübersicht für GAPDHS Antikörper (ABIN94395)
Target
Alle GAPDHS Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Verwendungszweck
- Anti-GAPDHS Purified
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Kreuzreaktivität (Details)
- Human, Porcine
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Produktmerkmale
- The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.
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Aufreinigung
- Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
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Reinheit
- > 95 % (by SDS-PAGE)
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Immunogen
- Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
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Isotyp
- IgM
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anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 1-408) antibody
GAPDHS Reaktivität: Human WB, ELISA, IHC (p) Wirt: Maus Monoclonal 2E3-2E10 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) antibodyGAPDHS Reaktivität: Human WB, IHC (p), IHC Wirt: Maus Monoclonal 1F6 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 104-134) antibodyGAPDHS Reaktivität: Human WB, IHC (p), FACS Wirt: Kaninchen Polyclonal RB16542 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 251-350) antibodyGAPDHS Reaktivität: Maus, Ratte WB, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 1-408) antibodyGAPDHS Reaktivität: Human WB, IP Wirt: Kaninchen Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 1-408) antibodyGAPDHS Reaktivität: Human WB Wirt: Maus Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (N-Term) antibodyGAPDHS Reaktivität: Human WB Wirt: Kaninchen Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 107-156) antibodyGAPDHS Reaktivität: Human, Maus, Affe, Schwein, Meerschweinchen, Pferd, Kaninchen WB Wirt: Kaninchen Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) antibody (FITC)GAPDHS Reaktivität: Human, Schwein FACS Wirt: Maus Monoclonal Hs-8 FITC
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) antibodyGAPDHS Reaktivität: Human WB, ELISA Wirt: Kaninchen Polyclonal unconjugated
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Applikationshinweise
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Immunocytochemistry: Recommended dilution: 10 μg/mL, membrane permeabilization (acetone) is essential.
Flow cytometry: Recommended dilution: 3-12 μg/mL. Intraacrosomal staining. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Do not freeze
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store at 2-8°C. Do not freeze.
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- GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))
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Andere Bezeichnung
- GAPDHS
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Hintergrund
- Glyceraldehyde-3-phosphate dehydrogenase, spermato,GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,SGAPDH, HSD-35
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Gen-ID
- 26330
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UniProt
- O14556
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Pathways
- Regulation of Carbohydrate Metabolic Process
Target
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