Dextranase Antikörper

Antigen: Dextranase

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN98392

Produktbeschreibung

Weitere Bezeichnung: Dextranase, Penicillium Species

Immunogen: Dextranase [Penicillium species]

Format: Lyophilized

Spezifität: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Dextranase [Penicillium species]. Cross reactivity against Dextranase from other tissues and species may occur but have not been specifically determined.

Anwendungen

Applikationshinweise: Recommended Dilutions: This product has been assayed against 1.0 ug of Dextranase [Penicillium species] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code ABIN101990 and (ABTS (2,2-azino-bis-[3-ethyl-benthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.

Konzentration: 90.0 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at 4° C prior to restoration. Restore with 2.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of restoration.

Forschungsgebiet: Enzyme

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Both the antiserum and IgG fractions of anti-Dextranase (Penicillium) are shown to detect under reducing conditions of SDS-PAGE the 41,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:2,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (ABIN101990) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.