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SLP65 / BLNK Antikörper
| Antigen | SLP65 / BLNK |
| Klonalität | Polyklonal |
| Wirt |
Kaninchen |
| Reaktivität |
Maus |
| Applikation |
Western Blot (WB)
|
| Produktnummer | ABIN94495 |
| Menge | 0.1 mg (1 mg/ml) |
| Preis | 230,00 € Zzgl. Versandkosten €20,00 und MWSt |
| Lieferung nach |
|
| Verfügbarkeit | Lieferung in 3 bis 5 Werktagen |
Produktbeschreibung
| Immunogen | A fusion protein representing amino acids 171-356 of human BLNK. |
| Format | Purified |
| Beschreibung | SLP65 / BLNK (SH2 domain-containing leukocyte-specific phosphoprotein of 65 kDa, B celllinker protein), also known as BASH, is an adaptor protein that plays key role in B cellactivation initiated by cross-linking the B cell receptor (BCR). Phosphorylated by Syktyrosine kinase, SLP65 serves as a scaffold for Btk tyrosine kinase, Vav1 guaninenucleotide exchange factor, phospholipase C gamma2, as well as Grb2 and Nck adaptorproteins, thus represents a central linker protein that bridges the BCR-associated kinaseswith a multitude of signaling pathways. |
| Spezifität | The polyclonal antibody reacts with mouse SLP65 / BLNK, a cytosolic adaptor proteinidentified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternativelyspliced forms of human SLP65). |
Anwendungen
| Applikationshinweise | Western Blotting. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withreducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions. |
| Konzentration | 1 mg/ml |
| Reinheit | > 95% (by SDS-PAGE) |
| Reinigung | Purified from rabbit serum by precipitation methods. |
| Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Lagerung | Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles. |
| Beschränkungen | Nur für Forschungszwecke einsetzbar |



