Spleen tyrosine Kinase (SYK) Antikörper

Antigen: Spleen tyrosine Kinase (SYK)

Synonyme: FLJ25043, FLJ37489, DKFZp313N1010, Syk, p72syk

Klonalität: Monoklonal (SYK-01)

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Immunpräzipitation (IP), Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunzytochemie (ICC)

Produktnummer: ABIN94475

Produktbeschreibung

Weitere Bezeichnung: Syk

Immunogen: Recombinant fragment (aa 5-360) of human Syk.

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: SYK-01

Beschreibung: Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane uponB cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, andphosphorylates downstream adaptor proteins, thereby providing docking sites for initiation ofsubsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, ortranscription of specific genes. Syk binds to the receptor assemblies through interactions ofits pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylatedby Src-family kinases. These kinases also help to activate Syk by phosphorylation of itsactivation loop.

Spezifität: The antibody SYK-01 reacts with protein tyrosine kinase p72Syk (Syk, Syk familytyrosine-specific phospho-transferase), which is required for the transduction of signalsthrough the B cell antigen receptor (BCR) and the high affinity IgE receptor (FcepsilonRI).

Anwendungen

Applikationshinweise: Western Blotting - Recommended dilution: 1-2 µg/ml, 60 min Positive control: RBL rat basophilic leukemia cell lineA-431 human epidermoid carcinoma cell lineRAMOS lymphoma cell lineU-937 human histiocytic lymphoma cell lineJURKAT human peripheral blood T cell leukemia cell line. Negative control: HeLa human cervix carcinoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold lysis buffer (1% laurylmaltoside in 20 mMTris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60min on ice. Centrifuge to remove cell debris. Mix lysate (1: 1) with non-reducing SDS-PAGEsample buffer. Application note: Non-reducing conditions. SDS-PAGE (12% separating gel). Immunohistochemistry (paraffin sections) - Recommended dilution: 5 µg/ml. Positive tissue: tonsil B cells

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Immunologie, Angeborene Immunität, Tyrosinkinasen, Signaltransduktion

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western Blotting analysis (non-reducing conditions) of whole cell lysate of RAMOS human Burkitt lymphoma cell line (1), RBL rat basophilic leukemia cell line (2) and HeLa human cervix carcinoma cell line (3, 4).Lane 1, 2: immunostaining with anti-Syk (SYK-01)Lane 3, 4: immunostaining with anti-human Cytokeratin 18 (DC-10, cat. no. 11-107-C100)Lane 5: negative control