HLA Class I Antikörper (Biotin)

Antigen: HLA Class I

Klonalität: Monoklonal (W6-32)

Wirt: Maus

Reaktivität: Human, Primaten, Rind (Kuh), Katze

Konjugat: Biotin

Produktnummer: ABIN94333

Produktbeschreibung

Weitere Bezeichnung: HLA-Class I

Immunogen: Membrane of human tonsil cells

Isotyp: IgG2a

Klon: W6-32

Beschreibung: HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteinsexpressed on nucleated cells and noncovalently associated with an invariant beta2microglobulin. They carry foreign determinants important for immune recognition bycytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class Iantigens are represented by HLA-A, HLA-B and HLA-C molecules.

Spezifität: The antibody W6/32 recognises MHC Class I molecules (MHC Class Ia) that are expressedon the surface of all human nucleated cell types.The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expressionin different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathyand other neuromuscular disorders.This antibody W6/32 is also suitable as a positive control for HLA tissue typing andcrossmatching. This antibody does not react with: Rabbit .

Anwendungen

Applikationshinweise: Biotinylated antibody is designed for indirect immunofluorescence analysis by FlowCytometry. Suggested working dilution is 1: 300.Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Konzentration: 1 mg/ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 Preparation: The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. Thereagent is free of unconjugated biotin..

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Haupthistokompatibilitätskomplex (MHC)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Surface staining of human peripheral blood cells with anti-HLA-class I (W6/32) Alexa Fluor® 488.