FYN Oncogene Related To SRC, FGR, YES (FYN) Antikörper

Antigen: FYN Oncogene Related To SRC, FGR, YES (FYN)

Synonyme: FYN, fyn, MGC52878, MGC132140, zgc:109996, c-fyn, zgc:86720, SLK, SYN, MGC45350, AI448320, AW552119, MGC115870, PKC

Klonalität: Monoklonal (FYN-01)

Wirt: Maus

Reaktivität: Human, Maus

Applikation: Immunpräzipitation (IP), Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunzytochemie (ICC)

Produktnummer: ABIN94311

Produktbeschreibung

Weitere Bezeichnung: Fyn

Immunogen: Bacterially expressed recombinant fragment of human Fyn (aa 7-176).

Format: Purified

Isotyp: IgG2b  (Passende Sekundärantikörper)

Klon: FYN-01

Beschreibung: Fyn is a ubiquitously expressed Src-family protein tyrosine kinase with important roles e.g.in immune and nervous system. It regulates N-methyl-D-aspartate (NMDA) receptorfunctions, thus affecting various brain functions, and even many of its other substrates areimportant for neural migration, synaptic plasticity, oligodendrocyte differentiation, and axongrowth and guidance. In immune system Fyn namely regulates the commitment of T cells toactivation, is important in T cell anergy induction, promotes mast cell chemotaxis andreorganization of cytoskeleton and participates in mast cell activation. Fyn is also involved inembryonic stem cell growth and differentiation, associates with tubulin and may play roles inmitotic spindle formation.

Spezifität: The antibody FYN-01 reacts with Fyn, a 59 kDa non-receptor Src-family protein tyrosinekinase.

Anwendungen

Applikationshinweise: Immunoprecipitation - Positive control: J77 T cell line,Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer withN-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1% laurylmaltosid (w/v),50 mM NaF). Application note: The antibody FYN-01 apparently gives very specific signal (by 59 kDa),and nicely immunoprecipitates Fyn from cell lysates. - Recommended dilution: 2 µg/ml. Positive control: JURKAT human T cell leukemia cell line. Immunohistochemistry (paraffin sections) - Recommended dilution: 5 µg/ml. Positive tissue: brainImmunocytochemistry

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from hybridoma culture supernatant by protein A-affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Krebs, Tyrosinkinasen, Alzheimer-Krankheit (Morbus Alzheimer), Signaltransduktion

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunoprecipitation of Fyn from the lysate of T cells isolated from fresh buffy coats. Western blot was immunostained with anti-Fyn (FYN-01). Lane 1: original lysate of T cellsLane 2-3: Immunoprecipitated material eluted from affinity sorbent (FYN-01 coupled to Sepharose beads). Lanes differ in amount of T cell lysate loaded on the immunosorbent.

Publikationen

Publikationen: Queval, Nicolas, Beau: "Role of Src kinases in mobilization of glycosylphosphatidylinositol-anchored decay-accelerating factor by Dr fimbria-positive adhering bacteria." in: Infection and immunity, Vol. 79, Issue 7, pp. 2519-34, 2011 (PubMed).