CD33 Molecule (CD33) Antikörper (Biotin)

Antigen: CD33 Molecule (CD33)

Synonyme: CD33, p67, SIGLEC3, FLJ00391, SIGLEC-3

Klonalität: Monoklonal (HIM3-4)

Wirt: Maus

Reaktivität: Human, Primaten

Konjugat: Biotin

Produktnummer: ABIN94073

Produktbeschreibung

Weitere Bezeichnung: CD33

Immunogen: NFMY-9s human cell line

Isotyp: IgG1

Klon: HIM3-4

Beschreibung: CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin(Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif(ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 andSHP-2 to signal assemblies, these ITIMs are also used for ubiquitin-mediated removal of thereceptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, bindssialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target foracute myeloid leukemia.

Spezifität: The antibody HIM3-4 reacts with CD33, a 67 kDa type I transmembrane glycoprotein(immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes,dendritic cells and mast cells, it is absent on platelets, lymphocytes, erythrocytes andhematopoietic stem cells.HLDA V, WS Code M MA112HLDA VI, WS Code M MA47

Anwendungen

Applikationshinweise: Biotinylated antibody is designed for indirect immunofluorescence analysis by FlowCytometry. Suggested working dilution is 1: 1000.Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Konzentration: 1 mg/ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Stammzellen, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Bonnet: "Humanized model to study leukemic stem cells." in: Methods in molecular biology (Clifton, N.J.), Vol. 538, pp. 247-62, 2009 (PubMed).

Hegde, Jankowska-Gan, Roenneburg et al.: "Human NKT cells promote monocyte differentiation into suppressive myeloid antigen-presenting cells." in: Journal of leukocyte biology, Vol. 86, Issue 4, pp. 757-68, 2009 (PubMed).