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Antikörper
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anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) Antikörper (FITC)
Platelet/endothelial Cell Adhesion Molecule (PECAM1) Antikörper (FITC)
| Antigen |
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| Synonyme |
CD31, PECAM-1, FLJ58394, Cd31, Pecam, C85791, MGC102160, MGC124998, PECAM1 |
| Klonalität |
Monoklonal (MEM-05) |
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Wirt
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Reaktivität
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Human (208), Maus (136), Ratte (Rattus) (61), Hund (35), Schwein (35), Pferd (18), Kaninchen (18), Affe (9), Rind (Kuh) (8), Schaf (7), Huhn (2), Ziege (1)
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Konjugat
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FITC (37), PE (27), Biotin (26), APC (14), Alexa Fluor 488 (9), Alexa Fluor 647 (8), PE,Cy7 (8), RPE (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), EFluor 605NC (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), RBITC (2), Alexa Fluor 700 (1), PE,DY590 (1), PE-Dyomics 590 (1), Pacific Blue (1), PerCP (1)
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Applikation
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Durchflusszytometrie (FACS) (259), Western Blot (WB) (82), Immunfluoreszenz (IF) (70), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (62), Immunhistochemie (Paraffinschnitte) (IHC (p)) (58), Immunpräzipitation (IP) (50), Immunhistochemie (IHC) (32), ELISA (17), Enzyme Immunoassay (EIA) (17), Funktionale Studien (Func) (7), Blockierende Antikörper (Inhibition) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (5), ELISA (Detektionsantikörper) (3), Dot Blot (Dot) (2), Immunoassay (IA) (1), Immunelektronenmikroskopie (EM) (1), Immunoelectron Microscopy (IEM) (1)
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| Produktnummer |
ABIN94071 |
| Menge |
100 tests |
| Preis |
230,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 3 bis 5 Werktagen |
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Weitere Bezeichnung
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CD31
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Immunogen
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Leukocytes of patient suffering from LGL-type leukaemia
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Isotyp
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IgG1
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Klon
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MEM-05
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Beschreibung
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CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptorinvolved in rebulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-basedinhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation providedocking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within thevascular compartment and is located mainly at junctions between adjacent cells. N-terminalIg-like domain of CD31 is responsible for its homophilic binding, which plays an importantrole in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles inmodulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis,apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophagephagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatorymolecules in vascular system.
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Spezifität
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The antibody MEM-05 reacts with CD31 (PECAM-1), a 130-140 kDa type I transmembraneglycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stemcells of the myeloid lineage.
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Applikationshinweise
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The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
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Buffer
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The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
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Lagerung
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Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
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Forschungsgebiet
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Stammzellen, Hämopoetische Vorläufer, Artherosklerose, Angiogenese, CD Antigene, Oberflächenrezeptoren der Immunzellen
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Publikationen
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Distler, Souady, Huelsewig et al.: "Shiga toxin receptor Gb3Cer/CD77: tumor-association and promising therapeutic target in pancreas and colon cancer." in: PLoS ONE, Vol. 4, Issue 8, pp. e6813, 2009 (PubMed).
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Alternativen zu Antigen "Platelet/endothelial Cell Adhesion Molecule (PECAM1)", Typ "Antikörper" finden
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Wirte
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Maus (179), Ratte (80), Kaninchen (70), Hamster (3), Schaf (1)
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Reaktivitäten
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Human (208), Maus (136), Ratte (Rattus) (61), Hund (35), Schwein (35), Pferd (18), Kaninchen (18), Affe (9), Rind (Kuh) (8), Schaf (7), Huhn (2), Ziege (1)
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Applikationen
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Durchflusszytometrie (FACS) (259), Western Blot (WB) (82), Immunfluoreszenz (IF) (70), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (62), Immunhistochemie (Paraffinschnitte) (IHC (p)) (58), Immunpräzipitation (IP) (50), Immunhistochemie (IHC) (32), ELISA (17), Enzyme Immunoassay (EIA) (17), Funktionale Studien (Func) (7), Blockierende Antikörper (Inhibition) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (5), ELISA (Detektionsantikörper) (3), Dot Blot (Dot) (2), Immunoassay (IA) (1), Immunelektronenmikroskopie (EM) (1), Immunoelectron Microscopy (IEM) (1)
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Konjugate
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FITC (37), PE (27), Biotin (26), APC (14), Alexa Fluor 488 (9), Alexa Fluor 647 (8), PE,Cy7 (8), RPE (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), EFluor 605NC (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), RBITC (2), Alexa Fluor 700 (1), PE,DY590 (1), PE-Dyomics 590 (1), Pacific Blue (1), PerCP (1)
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Epitope
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C-Term (13), pTyr713 (1)
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Alternativen 
