CD2 Molecule (CD2) Antikörper

Antigen: CD2 Molecule (CD2)

Synonyme: T11, SRBC, FLJ46032, CD2, MGC140284, CD2R, LFA2, OX34, LFA-2, OX-34, Ly37, Ly-37

Klonalität: Monoklonal (MEM-65)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP)

Produktnummer: ABIN94021

Produktbeschreibung

Weitere Bezeichnung: CD2

Gen-ID: 1p13

Immunogen: Human peripheral T cells.

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: MEM-65

Beschreibung: CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interactionwith CD58 stabilizes adhesion between T cells and antigen presenting or target cells.Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within thetwo-dimensional cell-cell interface to provide tight adhesion. Morover, T cell activationinduces increased CD2 expression and its lateral mobility, making easier contact betweenCD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites ofcell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reducesintestinal inflammation and helps to control infection.

Spezifität: The antibody MEM-65 recognizes an unique epitope of CD2, a 50 kDa glycoprotein presenton the human peripheral blood T-lymphocytes and NK cells, also expressed by allthymocytes.HLDA VI, WS Code T 6T-012

Anwendungen

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Stammzellen, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, Adaptive Immunität, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Ilangumaran, Briol, Hoessli: "CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes." in: Blood, Vol. 91, Issue 10, pp. 3901-8, 1998 (PubMed).

Espagnolle, Depoil, Zaru et al.: "CD2 and TCR synergize for the activation of phospholipase Cgamma1/calcium pathway at the immunological synapse." in: International immunology, Vol. 19, Issue 3, pp. 239-48, 2007 (PubMed).

Muhammad, Schiller, Forster et al.: "Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 12, pp. 7672-80, 2009 (PubMed).