Tubulin, alpha (TUBA) Antikörper

Antigen: Tubulin, alpha (TUBA)

Synonyme: Tuba1, LOC397931, MGC82007, Tua1, bmtua1, Tua2, bmtua2, Tua3, bmtua3, ALPHA TUBULIN, T29J13.200, TUA5, K-ALPHA-1, 4t, ALPHA 67C, D.m.ALPHA-67C, DTA2, Fs(3)Kav, Fs(3)Kavar, Fs(3)Sz14, Fs(3)Sz22, Fs(3)Tom, Fs(3)Tomaj, Kavar, T, Tom, Tub, aTub67C, alpha-Tub, alpha-Tub67C, alpha-tubulin, alpha-tubulin[4], alpha4, alpha4-t, alpha4-tubulin, alpha4Tub67C, alpha4t, alpha67C, alphaTUB, alphaTub4, alphaTub[67C], alphatub67C, mum-tub67C, DmelCG8308, CG8308, 1t, ALPHA 84C, BEST:LD32507, DM1alpha, DTA1, Talpha1, Tuba84B, Tubalpha1, a1t, aTub84B, agr;Tub, alpha-Tub84B, alpha1, alpha1t, alpha1tub, alpha84B, alphaT, alphaTub1, alphat-1, anon-EST:Liang-1.59, anon-EST:Liang-2.30, clone 1.59, clone 2.30, l(3)84Bd, l(3)g3, ms(3)nc33, nc33, tubulin, DmelCG1913, CG1913, 3t, ALPHA 84D, D.m.ALPHA-84D, DTA4, aTub84D, alpha-Tub84D, alpha3, alpha3t, alpha84D, alphaTub3, DmelCG2512, CG2512, 2t, ALPHA 85E, DTA3, a2t, aTub85E, alpha-Tub85E, alpha2, alpha2t, alpha85E, alpha85E-tub, alphaTub2, alphaTub84E, DmelCG9476, CG9476, TUBA3E, TUBA2, LIS3, TUBA3, FLJ25113, B-ALPHA-1, Tuba3b, Tuba3a, RGD1565155, TUBA3D, MGC133894, tua3

Klonalität: Monoklonal (TU-16)

Wirt: Maus

Reaktivität: Diverse Spezies

Applikation: Immunpräzipitation (IP), Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p)), ELISA, Immunzytochemie (ICC)

Produktnummer: ABIN93894

Produktbeschreibung

Weitere Bezeichnung: alpha-tubulin

Immunogen: Porcine brain microtubule protein MTP-1.

Format: Purified

Isotyp: IgM  (Passende Sekundärantikörper)

Klon: TU-16

Beschreibung: The microtubules are intracellular dynamic polymers made up of evolutionarily conservedpolymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associatedproteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamicminus ends. Microtubules are required for vital processes in eukaryotic cells includingmitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are alsonecessary for movement of cells by means of flagella and cilia. In mammalian tissue culturecells microtubules have their minus ends anchored in microtubule organizing centers(MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimerto associate with other heterodimers to form microtubule. In vivo, microtubule dynamics varyconsiderably. Microtubule polymerization is reversible and a populations of microtubules incells are on their minus ends either growing or shortening this phenomenon is calleddynamic instability of microtubules. On a practical level, microtubules can easily bestabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or morecommonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at roomtemperature for many hours. Using limited proteolysis by enzymes both tubulin subunits canbe divided into N-terminal and C-terminal structural domains.The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that existsin cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. Indifferent species it is coded by multiple tubulin genes that form tubulin classes (in human 6genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypesare expressed ubiquitously, while some have more restricted tissue expression.Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypesand their posttranslational modifications are responsible for multiple tubulin charge variants -tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structuraldomain.

Spezifität: The antibody TU-16 reacts with alpha-tubulin of all tested species, under denaturing andnon-denaturing conditions.

Synonyme: Tuba1, LOC397931, MGC82007, Tua1, bmtua1, Tua2, bmtua2, Tua3, bmtua3, ALPHA TUBULIN, T29J13.200, TUA5, K-ALPHA-1, 4t, ALPHA 67C, D.m.ALPHA-67C, DTA2, Fs(3)Kav, Fs(3)Kavar, Fs(3)Sz14, Fs(3)Sz22, Fs(3)Tom, Fs(3)Tomaj, Kavar, T, Tom, Tub, aTub67C, alpha-Tub, alpha-Tub67C, alpha-tubulin, alpha-tubulin[4], alpha4, alpha4-t, alpha4-tubulin, alpha4Tub67C, alpha4t, alpha67C, alphaTUB, alphaTub4, alphaTub[67C], alphatub67C, mum-tub67C, DmelCG8308, CG8308, 1t, ALPHA 84C, BEST:LD32507, DM1alpha, DTA1, Talpha1, Tuba84B, Tubalpha1, a1t, aTub84B, agr;Tub, alpha-Tub84B, alpha1, alpha1t, alpha1tub, alpha84B, alphaT, alphaTub1, alphat-1, anon-EST:Liang-1.59, anon-EST:Liang-2.30, clone 1.59, clone 2.30, l(3)84Bd, l(3)g3, ms(3)nc33, nc33, tubulin, DmelCG1913, CG1913, 3t, ALPHA 84D, D.m.ALPHA-84D, DTA4, aTub84D, alpha-Tub84D, alpha3, alpha3t, alpha84D, alphaTub3, DmelCG2512, CG2512, 2t, ALPHA 85E, DTA3, a2t, aTub85E, alpha-Tub85E, alpha2, alpha2t, alpha85E, alpha85E-tub, alphaTub2, alphaTub84E, DmelCG9476, CG9476, TUBA3E, TUBA2, LIS3, TUBA3, FLJ25113, B-ALPHA-1, Tuba3b, Tuba3a, RGD1565155, TUBA3D, MGC133894, tua3

Anwendungen

Applikationshinweise: Immunoprecipitation. Application note: reducing conditions. Western Blotting - Recommended dilution: 1-2 µg/ml, 60 min Positive control: HPB-ALL human peripheral blood leukemia cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withreducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions. Immunohistochemistry (paraffin sections) - Recommended dilution: 10 µg/ml

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from ascites by precipitation methods.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. Do not freeze.

Forschungsgebiet: Zytoskelett

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of alpha tubulin is around 50 kDa.L = lysateIPr = immunoprecipitate (reducing conditions)IPn = immunoprecipitate (non-reducing conditions)