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anti-5 bromodeoxyuridine (BrdU) antibody

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Antigen: 5 bromodeoxyuridine (BrdU)
Klonalität: Monoklonal (MOBU-1)
Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC)
Reaktivität: All Species
Wirt: Mouse
8 Publikationen vorhanden 8 Publikationen vorhanden
Menge: 0.1 mg (1 mg/ml) (Produktvarianten)
Preis: 328,12 $   (Zzgl. Versandkosten $25 und MWSt, Invoice in EUR)
Bestellnummer: ABIN93883
Verfügbarkeit: Versandbereit innerhalb von 1 bis 3 Werktagen
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Produktbeschreibung  » anti-5 bromodeoxyuridine (BrdU) antibody

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Antigen 5 bromodeoxyuridine (BrdU)
Immunogen 5-bromodeoxyuridine conjugated with hemocyanine.
Reaktivität All Species
Klonalität Monoklonal
Format Purified
Isotyp IgG1 »Passende Sekundärantikörper
Beschreibung Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.
Klon MOBU-1
Wirt Mouse
Spezifität The antibody MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. The antibody MoBu-1 is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. It reacts also specifically with 5-bromouridine (BrU).

Anwendungen  » anti-5 bromodeoxyuridine (BrdU) antibody

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Applikation Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC)
» Hier finden Sie 6 Protokolle zu den gelisteten Anwendungen
Applikationshinweise Immunocytochemistry: 2 µg/ml. Works very well for Immunohistochemistry with paraffin embedded sections.
Konzentration 1 mg/ml
Reinheit > 95% (by SDS-PAGE)
Reinigung Purified from ascites by protein-A affinity chromatography.
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Lagerung Store at 2-8
Forschungsgebiet Cell Cycle
Beschränkungen Nur für Forschungszwecke einsetzbar

Publikationen  » anti-5 bromodeoxyuridine (BrdU) antibody

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Publikationen Schielen, Van Der Pijl, Bleumink et al.: "Local popliteal lymph node reactions to hexachlorobenzene and pentachlorobenzene: comparison with systemic effects." In: Immunopharmacology , Vol. 31, Issue 02. Mrz, pp. 171-81, 1997 (PubMed)

Peters, Ganesh, Kuipers et al.: "Epithelial cell proliferative activity of Barrett's esophagus: methodology and correlation with traditional cancer risk markers." In: Digestive diseases and sciences , Vol. 43, Issue 7, pp. 1501-6, 1998 (PubMed)

Buckiovua, Kubuinovua, Soukup et al.: "Hyperthermia in the chick embryo: HSP and possible mechanisms of developmental defects." In: The International journal of developmental biology , Vol. 42, Issue 5, pp. 737-40, 1998 (PubMed)

Stanek, Kiss, Raska: "Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription." In: European journal of cell biology , Vol. 79, Issue 3, pp. 202-7, 2000 (PubMed)

Peters, Ganesh, Kuipers et al.: "Effect of elimination of acid reflux on epithelial cell proliferative activity of Barrett esophagus." In: Scandinavian journal of gastroenterology , Vol. 35, Issue 12, pp. 1238-44, 2001 (PubMed)

Swee, Bosco, Malissen et al.: "Expansion of peripheral naturally occurring T regulatory cells by Fms-like tyrosine kinase 3 ligand treatment." In: Blood , Vol. 113, Issue 25, pp. 6277-87, 2009 (PubMed)

Gamper, Koh, Ruau et al.: "GAR22: a novel target gene of thyroid hormone receptor causes growth inhibition in human erythroid cells." In: Experimental hematology , Vol. 37, Issue 5, pp. 539-548.e4, 2009 (PubMed)

Johnson, Phua, Bennett et al.: "Studying vertebrate topoisomerase 2 function using a conditional knockdown system in DT40 cells." In: Nucleic acids research , Vol. 37, Issue 14, pp. e98, 2009 (PubMed)