ATP Synthase Subunit beta (AtpB) Antikörper

Antigen: ATP Synthase Subunit beta (AtpB)

Synonyme: ATPMB, ATPSB, MGC5231, ATP, ATPB, ATPase beta, ATPsyn b, ATPsyn-b, beta-ATPase, DmelCG11154, CG11154, ATP5B, atpmb, atpsb, MGC76033, im:6793121, wu:fj38d01, GLEAN_15322, NV12146, Atpsyn-beta

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Barley (Hordeum vulgare), Sojabohne (Glycine max), Tomate (Solanum lycopersicum), Reis (Oryza sativa), Populus, Spinat (Spinacia oleracea), Maize/Corn (Zea mays), Rind (Kuh), Huhn, Schwein, Ratte (Rattus), Lachs (Salmonidae), Seal (Pinnipedia)

Applikation: Western Blot (WB), Blue-native PAGE (BN PAGE)

Produktnummer: ABIN93568

Produktbeschreibung

Weitere Bezeichnung: Beta subunit of ATP synthase (AtpB)

Swiss-Prot: P19366

Immunogen: KLH-conjugated synthetic peptide derived from available plant, algal (chloroplasticand mitochondrial) and bacterial sequences of beta subunits of F-type ATPsynthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit betaAtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1At5g08670 as well as Chlamydomonas reinhardtii P06541 and A8IQU3

Format: Lyophilized

Beschreibung: ATP synthase is the universal enzyme that synthesizes ATP from ADP andphosphate using the energy stored in a transmembrane ion gradient.

Spezifität: Predicted reactivity: dicots, including Vitis vinifera, and monocots, algae, cyanobacteria, marinediatoms, bacteria including Yrsinia sp. Not reactive in: archeal V-type ATP synthase.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 2000 - 1: 5 000 with standard ECL (WB), 1: 5000 (BN-PAGE). Additional Information: the anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthasesubcomplex, as well as the chloroplastic CF1 Atp Synthase, and most knownbacterial F-type Atp Synthases. Peptide used for antibody product.

Reinheit: Serum

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

2 µg of total protein extracted with PEB from leaf tissue of (1) Arabidopsis thaliana, (2) Spinacia oleracea, (3) Lycopersicon esculentum, (4) Glycine max, (5) Populus sp., (6) Zea mays and (7) Hordeum vulgare were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. In parallel a dilution row (a-g: 10 - 5 - 2.5 - 1.25 - 0.63 - 0.32 - 0.16 µg protein/lane) from sample 1 (Arabidopsis) was processed. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-AtpB (1:5000, 1h) and secondary anti-rabbit (1:10000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (Invitrogen) using a Fuji LAS-3000 CCD (300s, standard sensitivity).

2 µg of total protein from (1) cow, (2) chicken, (3) pig, (4) rat, (5) salmon, (6) seal, (8) Arabidopsis thaliana, (9) Zea mays extracted with Protein Extration Buffer, PEB and separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 50 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (Agrisera anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 30 seconds.

Publikationen

Publikationen: Brown, Campbell, Lawrence: "Resource dynamics during infection of Micromonas pusilla by virus MpV-Sp1." in: Environmental microbiology, Vol. 9, Issue 11, pp. 2720-7, 2007 (PubMed).

Ahsan, Nakamura, Komatsu: "Differential responses of microsomal proteins and metabolites in two contrasting cadmium (Cd)-accumulating soybean cultivars under Cd stress." in: Amino acids, 2010 (PubMed).

Schmied, Hedtke, Grimm: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." in: Journal of plant physiology, 2011 (PubMed).

Berto, DAdamo, Bergantino et al.: "The cyanobacterium Synechocystis sp. PCC 6803 is able to express an active [FeFe]-hydrogenase without additional maturation proteins." in: Biochemical and biophysical research communications, 2011 (PubMed).