Albumin Antikörper (HRP)

Produktnummer ABIN784429

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Albumin in WB, IF, ID, RIA und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für Albumin Antikörper (HRP) (ABIN784429)

Target: Albumin (ALB)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Albumin Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunodiffusion (ID), Radioimmunoassay (RIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Albumin Antikörper (HRP)

Spezifität: Tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal rat serum and purified rat albumin. One characteristic precipitin line is obtained against pooled normal rat serum using different antigen/antibody concentration ratio's. Precipitin lines against normal rat serum and purified rat albumin give a reaction of full identity. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has been tested for cross-reactivity by double radial immunodiffusion against several species sera with the following results: ++ mouse, guinea pig + monkey ± horse, human, swine - rabbit, sheep, goat, canine, chicken, bovine

Produktmerkmale: Molar Ratio: 1,7

Aufreinigung: DEAE-column Chromatography

Immunogen: Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from rat serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 1 mL sterile distilled water

Konzentration: 10 mg/mL

Buffer: PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.

Details zu Albumin

Target: Albumin (ALB)

Andere Bezeichnung: Albumin

Hintergrund: Synonyms: ALB, BSA, HSA, Serum Albumin

Gen-ID: 24186

NCBI Accession: NP_599153

Pathways: Lipid Metabolism