His Tag Antikörper

Produktnummer ABIN781760

Der Maus Monoklonal anti-His Tag Antikörper wird verwendet zum Nachweis von His Tag. Er wurde validiert für WB und EIA.

Kurzübersicht für His Tag Antikörper (ABIN781760)

Target: His Tag

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Klon: 16-002

Produktdetails anti-His Tag Antikörper

Sequenz: HHHHHH

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Purified, recombinant C-terminal His6-tagged protein expressed in E. coli

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS pH 7.4, 15 mM Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, store (in aliquots) at -20 °C to -70 °C.

Details zu His Tag

Target: His Tag

Andere Bezeichnung: 6xHistidine Epitope Tag

Substanzklasse: Tag

Hintergrund: 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag