NFKBIE Antikörper (pSer157)

Produktnummer ABIN710726

Der Kaninchen Polyklonal anti-NFKBIE Antikörper wird verwendet zum Nachweis von NFKBIE in Proben von Human. Er wurde validiert für WB, ELISA, IF (cc), IF (p), IHC (p) und IHC (fro).

Kurzübersicht für NFKBIE Antikörper (pSer157) (ABIN710726)

Target: NFKBIE (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFKBIE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-NFKBIE Antikörper

Bindungsspezifität: pSer157

Homologie: Human,Cow

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human NFKBIE around the phosphorylation site of Ser157

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu NFKBIE

Target: NFKBIE (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE))

Andere Bezeichnung: Ikb epsilon

Hintergrund:

Synonyms: NFKBIE phospho S157, IKB epsilon Ser157, IKB epsilon S, p-IKB epsilon Ser157, p-IKB epsilon S157, I kappa B epsilon, IkappaBepsilon, IkB E, IKBE, IKB epsilon, MGC72568, NF kappa B inhibitor epsilon, NF kappa BIE, NFkappa BIE, NFkappaB inhibitor epsilon, NFKBIE, Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilon, OTTHUMP00000016522, Slc35b2, solute carrier family 35, member B2.

Background: NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine). For some genes, activation requires NFKB interaction with other transcription factors, such as STAT, AP1 (JUN), and NFAT.

Gen-ID: 4794

Pathways: NF-kappaB Signalweg, Maintenance of Protein Location