Autophagy/beclin-1 Regulator 1 (AMBRA1) Antikörper (Biotin)

Antigen: Autophagy/beclin-1 Regulator 1 (AMBRA1)

Synonyme: Ambra1, AA474864, AV021921, mKIAA1736, A130023A14, RP23-273F8.1, 2310079H06Rik, D030051N19Rik, DCAF3, WDR94, FLJ20294, KIAA1736, MGC33725, AMBRA1, MGC128737

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus), Kaninchen, Hund, Pferd, Huhn

Konjugat: Biotin

Applikation: Western Blot (WB), ELISA, Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)), Durchflusszytometrie (FACS), Immunfluoreszenz (IF)

Produktnummer: ABIN704743

Produktbeschreibung

Weitere Bezeichnung: AMBRA1

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human AMBRA1 N-terminus

Format: Lyophilized

Wiederherstellung: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotyp: IgG

Beschreibung: Synonym: Activating molecule in beclin 1 regulated autophagy, Activating molecule in BECN1 regulated autophagy protein 1, Hypothetical protein FLJ20294, Hypothetical protein LOC55626, KIAA1736, MGC33725, WDR94.

Molekulargewicht: 143kDa

Kommentare:

AMBRA1 regulates autophagy and development of the nervous system. It is a large

previously unknown protein bearing a WD40 domain at its amino terminus

regulates autophagy and has a crucial role in embryogenesis. AMBRA1 is a positive regulator of the Becn1-dependent programme of autophagy

as revealed by its overexpression and by RNA interference experiments invitro. Notably

AMBRA1 functional deficiency in mouse embryos leads to severe neural tube defects associated with autophagy impairment

accumulation of ubiquitinated proteins

unbalanced cell proliferation and excessive apoptotic cell death.

Anwendungen

Applikationshinweise: WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Konzentration: 1mg/1ml

Reinigung: Antibodies are purified by protein A and peptide affinity chromatography.

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Lagerung: Ship at room temperature. Store at -20°C

Forschungsgebiet: Krebs, Autophagie, Neurogenese

Beschränkungen: Nur für Forschungszwecke einsetzbar