WNT5A Antikörper (AA 301-381)

Produktnummer ABIN675758

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-WNT5A Antikörper wird verwendet zum Nachweis von WNT5A in Proben von Human, Maus, Ratte und Hund. Er wurde validiert für WB, ELISA, FACS, IHC (p), IF (cc), IF (p) und IHC (fro).

Kurzübersicht für WNT5A Antikörper (AA 301-381) (ABIN675758)

Target: WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WNT5A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-WNT5A Antikörper

Bindungsspezifität: AA 301-381

Kreuzreaktivität: Hund, Human, Maus, Ratte

Homologie: Cow,Pig,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human WNT5A

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-WNT5A Antikörper (ABIN675758)

Guo, Yi, Li, Fu, Li: "Snail1 is positively correlated with atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease." in: Experimental and therapeutic medicine, Vol. 14, Issue 5, pp. 4231-4237, (2017) (PubMed).

Saha, Aranda, Hayakawa, Bhanja, Atay, Brodin, Li, Asfaha, Liu, Tailor, Zhang, Godwin, Tome, Wang, Guha, Pollard: "Macrophage-derived extracellular vesicle-packaged WNTs rescue intestinal stem cells and enhance survival after radiation injury." in: Nature communications, Vol. 7, pp. 13096, (2016) (PubMed).

Król, Mucha, Majchrzak, Homa, Bulkowska, Majewska, Gajewska, Pietrzak, Perszko, Romanowska, Paw?owski, Manuali, Hellmen, Motyl: "Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, (2014) (PubMed).

Details zu WNT5A

Target: WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))

Andere Bezeichnung: WNT5A

Hintergrund:

Synonyms: hWNT5A, Protein Wnt-5a, WNT5A

Background: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.

Gen-ID: 7474

UniProt: P41221

Pathways: WNT Signalweg, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size, Tube Formation