GRP78 Antikörper (C-Term)

Produktnummer ABIN651231

Zitiert in 1 Publikation. Der Kaninchen Polyklonal anti-GRP78 Antikörper (Klon RB20814) (ABIN651231) detektiert spezifisch GRP78 in WB, FACS und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für GRP78 Antikörper (C-Term) (ABIN651231)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRP78 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB20814

Produktdetails anti-GRP78 Antikörper

Bindungsspezifität: AA 541-568, C-Term

Homologie: B, Ha, M, Pig, Rat, X

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 541-568 amino acids from the C-terminal region of human HSPA5.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-GRP78 Antikörper (ABIN651231)

Haenig, Atias, Taylor, Mazza, Schaefer, Russ, Riechers, Jain, Coughlin, Fontaine, Freibaum, Brusendorf, Zenkner, Porras, Stroedicke, Schnoegl, Arnsburg, Boeddrich, Pigazzini, Heutink, Taylor et al.: "Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains. ..." in: Cell reports, Vol. 32, Issue 7, pp. 108050, (2020) (PubMed).

Details zu GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Andere Bezeichnung: HSPA5

Hintergrund: When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.

Molekulargewicht: 72333

Gen-ID: 3309

NCBI Accession: NP_005338

UniProt: P11021

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling