HUWE1 Antikörper (C-Term)

Produktnummer ABIN499331

Der Kaninchen Polyklonal anti-HUWE1 Antikörper wird verwendet zum Nachweis von HUWE1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IF und EIA.

Kurzübersicht für HUWE1 Antikörper (C-Term) (ABIN499331)

Target: HUWE1 (HECT, UBA and WWE Domain Containing 1, E3 Ubiquitin Protein Ligase (HUWE1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HUWE1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Produktdetails anti-HUWE1 Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody detects ARF-BP1 at C-term.

Aufreinigung: Affinity chromatography purified via peptide column

Immunogen: A 19 amino acid peptide from near the carboxy terminus of Human ARF-BP1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot: ARF-BP1 antibody can be used for detection of ARF-BP1 at 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu HUWE1

Target: HUWE1 (HECT, UBA and WWE Domain Containing 1, E3 Ubiquitin Protein Ligase (HUWE1))

Andere Bezeichnung: HUWE1 / UREB1

Hintergrund: The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53, this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.Synonyms: ARF-BP1, ARF-binding protein 1, E3 ubiquitin-protein ligase HUWE1, HECT, HSPC272, HectH9, Homologous to E6AP carboxyl terminus homologous protein 9, KIAA0312, KIAA1578, LASU1, Large structure of UREB1, Mcl-1 ubiquitin ligase E3, Mule, UBA and WWE domain-containing protein 1, URE-B1, URE-binding protein 1, Upstream regulatory element-binding protein 1

Gen-ID: 10075

NCBI Accession: NP_113584

UniProt: Q7Z6Z7