FOXO4 Antikörper (pSer197)

Produktnummer ABIN498704

Der Kaninchen Polyklonal anti-FOXO4 Antikörper wird verwendet zum Nachweis von FOXO4 in Proben von Human und Maus. Er wurde validiert für WB und IHC (p).

Kurzübersicht für FOXO4 Antikörper (pSer197) (ABIN498704)

Target: FOXO4 (Forkhead Box O4 (FOXO4))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FOXO4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-FOXO4 Antikörper

Bindungsspezifität: pSer197

Spezifität: This antibody detects endogenous levels of AFX1 protein only when phosphorylated at Ser197.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu FOXO4

Target: FOXO4 (Forkhead Box O4 (FOXO4))

Andere Bezeichnung: FOXO4 / AFX1 / MLLT7

Hintergrund: FKHR (for forkhead in rhabdomyosarcoma), FKHRL1, and AFX1 are members of a subfamily of the forkhead family of transcription factors. AFX1, also known as FoxO4, is expressed in a wide variety of tissues and, like other FKHR proteins, AFX1 contains a single forkhead domain and serineproline-rich region, which mediate DNA binding. AFX1-mediated transcriptional activation is regulated by the serine/threonine kinase Akt1, which phosphorylates AFX1 (in humans, this phosphorylation can occur at several sites, including Ser 197) and in turn, sequesters AFX1 in the cytosol, thereby blocking nuclear localization and DNA binding. Genetic mutations in FKHR genes, including the t(2,13) and t(1,3) translocations, are commonly found in alveolar rhabdomyosarcomas. Additionally, the t(x,11) translocation of the AFX1 gene, which involves the fusion of a serine-prolinerich sequence of AFX1 to the carboxyterminus of a truncated MLL, results in acute lymphocytic leukemia.Synonyms: Fork head domain transcription factor AFX1, Forkhead box protein O4

Molekulargewicht: approx. 55, 70 kDa

Gen-ID: 4303

NCBI Accession: NP_005929

UniProt: P98177

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Muscle Cell Differentiation