BARD1 Antikörper

Produktnummer ABIN498379

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch BARD1 in WB und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für BARD1 Antikörper (ABIN498379)

Target: BARD1 (BRCA1 Associated RING Domain 1 (BARD1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BARD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-BARD1 Antikörper

Spezifität: This antibody detects endogenous levels of BARD1 protein. (region surrounding Arg8)

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu BARD1

Target: BARD1 (BRCA1 Associated RING Domain 1 (BARD1))

Andere Bezeichnung: BARD1

Hintergrund: Mutations within the BRCA1 gene, localized to chromosome 17q, are believed to account for approximately 45 % of families with increased incidence of both early-onset breast cancer and ovarian cancer. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1863 amino acids. This protein contains a RING domain near the N-terminus and appears to encode a tumor suppressor. BARD1 (BRCA1-associated RING domain protein 1) and BAP1 (BRCA1-associated protein 1) have both been shown to bind to the N-terminus of BRCA1 and are potential mediators of tumor suppression. BARD1 contains an N-terminal RING domain and three tandem ankyrin repeats. The C-terminus of BARD1 contains a region with sequence homology to BRCA1, termed the BRCT domain. BAP1 is an ubiquitin hydrolase and has been shown to enhance BRCA1-mediated cell growth suppression.Synonyms: BARD-1, BRCA1-associated RING domain protein 1

Molekulargewicht: approx. 85 kDa

Gen-ID: 580

NCBI Accession: NP_000456

UniProt: Q99728

Pathways: DNA Reparatur