DGKE Antikörper

Produktnummer ABIN498319

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch DGKE in WB und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für DGKE Antikörper (ABIN498319)

Target: DGKE (Diacylglycerol Kinase, epsilon 64kDa (DGKE))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DGKE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-DGKE Antikörper

Spezifität: This antibody detects endogenous levels of DGK-ε protein. (region surrounding Ser194)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography using epitope-specific immunogen

Reinheit: > 95 % (by SDS-PAGE)

Anwendungsinformationen

Applikationshinweise: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu DGKE

Target: DGKE (Diacylglycerol Kinase, epsilon 64kDa (DGKE))

Andere Bezeichnung: DAG Kinase epsilon

Hintergrund: Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-α (80 kDa), -β (90 kDa), and -γ (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK-δ (130 kDa) and DGK-?(130 kDa) contain N-terminal pleckstrin homology (PH) domains and are classified as type II. Human DGK-ε (64 kDa) contains no identifiable regulatory domains and is classified as a type III DGK. Human DGK-ζ (104 kDa) and -iota (130 kDa) possess C-terminal ankyrin repeats and are classified as type IV DGKs. Human DGK-θ (110 kDa) contains 3 cysteine-rich domains and a PH domain and is classified as a type V DGK.Synonyms: DAGK5, DGK-epsilon, DGKE, Diacylglycerol kinase epsilon, Diglyceride kinase epsilon

Molekulargewicht: approx. 60 kDa

Gen-ID: 8526

NCBI Accession: NP_003638

UniProt: P52429