MYB Antikörper (Internal Region)

Produktnummer ABIN498072

Der Ziege Polyklonal anti-MYB Antikörper (ABIN498072) detektiert spezifisch MYB in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Hund, Rind (Kuh) und Schwein.

Kurzübersicht für MYB Antikörper (Internal Region) (ABIN498072)

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MYB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-MYB Antikörper

Bindungsspezifität: AA 557-569, Internal Region

Sequenz: C-HRDQTVKTQK ENT

Spezifität: This antibody is expected to recognize all reported isofoms (NP_001123645.1, NP_005366.2, NP_001123644.1, NP_001155128.1, NP_001155129.1, NP_001155130.1, NP_001155131.1, NP_001155132.1).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine, Pig.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide corresponding to internal region of Human c-Myb Genename: MYB

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit: 1/8000. Western blot: 0.5-1.5 μg/mL. Detects a band of Approx 85 kDa in lysates of cell line Jurkat.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu MYB

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Andere Bezeichnung: MYB

Hintergrund: Myb is a transcriptional activator, DNA-binding protein that recognizes the sequence 5'-YAAC[GT]G-3'. It participates in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed predominantly in haematopoietic cells and immature gastroepithelial cells. TRAF7 stimulates the sumoylation of Myb at Lys-523 and Lys-499, which are the same sites as those used for PIASy-induced sumoylation. A correlation has been established between reduction in sumoylation of Myb and increase in transcriptional activation. Negative influence on transactivation properties by the negative regulatory domain region of c-Myb depends on upon sumoylation.Synonyms: Proto-oncogene c-Myb, Transcriptional activator Myb, c-Myb

Gen-ID: 4602

NCBI Accession: NP_005366

UniProt: P10242

Pathways: Chromatin Binding, Positive Regulation of Immune Effector Process