Nav1.8 Antikörper (C-Term)

Produktnummer ABIN492472

Dieser Maus Monoklonal Antikörper detektiert spezifisch Nav1.8 in WB, IF, IP und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Maus.

Kurzübersicht für Nav1.8 Antikörper (C-Term) (ABIN492472)

Target: Nav1.8 (SCN10A) (Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nav1.8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: S134-12

Produktdetails anti-Nav1.8 Antikörper

Bindungsspezifität: AA 1724-1956, C-Term

Spezifität: Detects ~220 kDa protein. No cross reactivity against other Nav channels.

Produktmerkmale: Synonyms: Sodium channel protein type 10 subunit alpha, Sodium channel protein type X subunitalpha, Voltage-gated sodium channel subunit alpha Nav1.8, Peripheral nerve sodiumchannel 3

Aufreinigung: Protein G Chromatography.

Immunogen: Fusion protein amino acids 1724-1956 (cytoplamic C-terminus) of Rat SCN10A/NAV1. 8

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western blot: 1 μg/mL (if results are poor, use the lysate in SDS buffer but without boiling,incubate for 20 mins at 37 °C).1 μg/mL was sufficient for detection of Nav1.8in 10 μg COS cell lysate transiently expressingNav1.8 by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as thesecondary antibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mLImmunohistochemistry: 0.1-1.0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Nav1.8

Target: Nav1.8 (SCN10A) (Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A))

Andere Bezeichnung: SCN10A / PN3

Hintergrund: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). NAV1.8 is a voltage-gated sodium channel and plays a critical role in the generation and conduction of action potentials and is thus important for electrical signaling by most excitable cells. Therapeutically, the association of pain insensitivity with the loss of function of a certain sodium channel may have implications. Since Nav1.8 is not present in cardiac muscle or neurons in the central nervous system, blockers of Nav1.8 will not have direct action on these cells and thus can have less side effects than current pain medications. By performing more studies, there is a possibility to develop a new generation of drugs that can reduce the pain intensity in animals (3).Synonyms: Peripheral nerve sodium channel 3, Sodium channel protein type 10 subunit alpha, Sodium channel protein type X subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.8

Gen-ID: 29571

UniProt: Q62968